Structure of PDB 1kmn Chain B

Receptor sequence
>1kmnB (length=364) Species: 562 (Escherichia coli) [Search protein sequence]
KNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLPIVEQTPLF
KRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWW
RALGISEHVTLELNSIGYLDEESREHFAGLCKLLESAGIAYTVNQRLVRG
LDYYNRTVFEWVTNSLGSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLE
RLVLLVQAVNPEFKADPVVDIYLVASGADTQSAAMALAERLRDELPGVKL
MTNHGGGNFKKQFARADKWGARVAVVLGESEVANGTAVVKDLRSGEQTAV
AQDSVAAHLRTLLG
3D structure
PDB1kmn The first step of aminoacylation at the atomic level in histidyl-tRNA synthetase.
ChainB
Resolution2.8 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 6.1.1.21: histidine--tRNA ligase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 HSO B E83 T85 Q127 E131 Y263 Y264 G304 F305 A306 E81 T83 Q125 E129 Y203 Y204 G244 F245 A246
BS02 ATP B R113 G120 R121 Y122 F125 Q127 R259 T281 V282 A306 G308 R311 R111 G118 R119 Y120 F123 Q125 R199 T221 V222 A246 G248 R251
Gene Ontology
Molecular Function
GO:0004812 aminoacyl-tRNA ligase activity
GO:0004821 histidine-tRNA ligase activity
GO:0005524 ATP binding
GO:0042803 protein homodimerization activity
Biological Process
GO:0006412 translation
GO:0006427 histidyl-tRNA aminoacylation
Cellular Component
GO:0005737 cytoplasm
GO:0005829 cytosol

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Cellular Component
External links
PDB RCSB:1kmn, PDBe:1kmn, PDBj:1kmn
PDBsum1kmn
PubMed9207058
UniProtP60906|SYH_ECOLI Histidine--tRNA ligase (Gene Name=hisS)

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