Structure of PDB 1kl2 Chain B

Receptor sequence

>1kl2B (length=405) Species: 1422 (Geobacillus stearothermophilus) [Search protein sequence]

MKYLPQQDPQVFAAIEQERKRQHAKIELIASENFVSRAVMEAQGSVLTNK
YAEGYPGRRYYGGCEYVDIVEELARERAKQLFGAEHANVQPHSGAQANMA
VYFTVLEHGDTVLGMNLSHGGHLTHGSPVNFSGVQYNFVAYGVDPETHVI
DYDDVREKARLHRPKLIVAAASAYPRIIDFAKFREIADEVGAYLMVDMAH
IAGLVAAGLHPNPVPYAHFVTTTTHKTLRGPRGGMILCQEQFAKQIDKAI
FPGIQGGPLMHVIAAKAVAFGEALQDDFKAYAKRVVDNAKRLASALQNEG
FTLVSGGTDNHLLLVDLRPQQLTGKTAEKVLDEVGITVNKNTIPYDPESP
FVTSGIRIGTAAVTTRGFGLEEMDEIAAIIGLVLKNVGSEQALEEARQRV
AALTD

3D structure

PDB

1kl2 Crystal structure of binary and ternary complexes of serine hydroxymethyltransferase from Bacillus stearothermophilus: insights into the catalytic mechanism.

Chain

Resolution

2.7 Å

3D
structure

Catalytic site residues are labeled in the structure
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Enzymatic activity

Catalytic site (original residue number in PDB)	Y51 E53 D197 T223 K226 R232
Catalytic site (residue number reindexed from 1)	Y51 E53 D197 T223 K226 R232
Enzyme Commision number	2.1.2.1: glycine hydroxymethyltransferase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)
BS01	GLY	B	Y51 Y61	Y51 Y61
BS02	FON	B	E53 Y60 F251	E53 Y60 F251
BS03	PLP	B	S93 G94 A95 H122 A171 S172 D197 A199 H200 T223 K226	S93 G94 A95 H122 A171 S172 D197 A199 H200 T223 K226
BS04	GLY	B	S31 H200 K226 R357	S31 H200 K226 R357
BS05	FON	B	L117 G120 G121 H122 S172 N341 P350 F351	L117 G120 G121 H122 S172 N341 P350 F351

Gene Ontology

	Molecular Function
GO:0003824	catalytic activity
GO:0004372	glycine hydroxymethyltransferase activity
GO:0008270	zinc ion binding
GO:0016740	transferase activity
GO:0030170	pyridoxal phosphate binding
GO:0050897	cobalt ion binding
GO:0070905	serine binding

	Biological Process
GO:0006545	glycine biosynthetic process
GO:0006565	L-serine catabolic process
GO:0006730	one-carbon metabolic process
GO:0008652	amino acid biosynthetic process
GO:0019264	glycine biosynthetic process from serine
GO:0035999	tetrahydrofolate interconversion
GO:0046653	tetrahydrofolate metabolic process
GO:0046655	folic acid metabolic process

	Cellular Component
GO:0005737	cytoplasm
GO:0005829	cytosol

View graph for
Molecular Function

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Biological Process

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Cellular Component

External links

PDB	RCSB:1kl2, PDBe:1kl2, PDBj:1kl2
PDBsum	1kl2
PubMed	11877399
UniProt	Q7SIB6

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