Structure of PDB 1kj8 Chain B

Receptor sequence
>1kj8B (length=384) Species: 562 (Escherichia coli) [Search protein sequence]
TLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRYADAPAMH
VAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNV
VPCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVADIGYP
CIVKPVMSSSGKGQTFIRSAEQLAQAWKYAQGRVIVEGVVKFDFEITLLT
VSAVDGVHFCAPVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLAL
GGYGLFGVELFVCGDEVIFSEVSPRPHDTGMVTLISQDLSEFALHVRAFL
GLPVGGIRQYGPAASAVILPQLTSQNVTFDNVQNAVGADLQIRLFGKPEI
DGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
3D structure
PDB1kj8 PurT-encoded glycinamide ribonucleotide transformylase. Accommodation of adenosine nucleotide analogs within the active site.
ChainB
Resolution1.6 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) E115 K155 S160 G162 E267 E279 D286 T287 R363
Catalytic site (residue number reindexed from 1) E114 K154 S159 G161 E259 E271 D278 T279 R355
Enzyme Commision number 6.3.1.21: phosphoribosylglycinamide formyltransferase 2.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 MG B E267 E279 E259 E271
BS02 ATP B R114 K155 S161 G162 Q165 E195 V198 E203 E267 F269 E279 R113 K154 S160 G161 Q164 E187 V190 E195 E259 F261 E271
BS03 GAR B G21 L23 E82 I83 D286 K355 R362 R363 G20 L22 E81 I82 D278 K347 R354 R355
Gene Ontology
Molecular Function
GO:0000287 magnesium ion binding
GO:0003824 catalytic activity
GO:0004644 phosphoribosylglycinamide formyltransferase activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0008776 acetate kinase activity
GO:0016742 hydroxymethyl-, formyl- and related transferase activity
GO:0016874 ligase activity
GO:0043815 phosphoribosylglycinamide formyltransferase 2 activity
GO:0046872 metal ion binding
Biological Process
GO:0006164 purine nucleotide biosynthetic process
GO:0006189 'de novo' IMP biosynthetic process
GO:0009152 purine ribonucleotide biosynthetic process
Cellular Component
GO:0005829 cytosol

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Molecular Function
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Cellular Component
External links
PDB RCSB:1kj8, PDBe:1kj8, PDBj:1kj8
PDBsum1kj8
PubMed11953435
UniProtP33221|PURT_ECOLI Formate-dependent phosphoribosylglycinamide formyltransferase (Gene Name=purT)

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