Structure of PDB 1k6m Chain B

Receptor sequence
>1k6mB (length=432) Species: 9606 (Homo sapiens) [Search protein sequence]
NSPTMVIMVGLPARGKTYISTKLTRYLNFIGTPTKVFNLGQYRREAVSYK
NYEFFLPDNMEALQIRKQCALAALKDVHNYLSHEEGHVAVFDATNTTRER
RSLILQFAKEHGYKVFFIESICNDPGIIAENIRQVKLGSPDYIDCDREKV
LEDFLKRIECYEVNYQPLDEELDSHLSYIKIFDVGTRYMVNRVQDHIQSR
TVYYLMNIHVTPRSIYLCRHGESELNIRGRIGGDSGLSVRGKQYAYALAN
FIQSQGISSLKVFTSRMKRTIQTAEALGVPYEQFKALNEIDAGVCEEMTY
EEIQEHYPEEFALRDQDKYRYRYPKGESYEDLVQRLEPVIMELERQENVL
VICHQAVMRCLLAYFLDKSSEELPYLKCPLHTVLKLTPVAYGCKVESIYL
NVEAVNTHREKPENVDITREPEEALDTVPAHY
3D structure
PDB1k6m Tissue-specific structure/function differentiation of the liver isoform of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase.
ChainB
Resolution2.4 Å
3D
structure
Catalytic site residues are labeled in the structure
[Spin on]
[Spin off]
[Reset orientation]

[High quality]
[Low quality]

[White background]
[Black background]

[Download]
[Download structure with residue number starting from 1]
Enzymatic activity
Catalytic site (original residue number in PDB) R257 H258 N264 R307 E327 H392
Catalytic site (residue number reindexed from 1) R219 H220 N226 R269 E289 H354
Enzyme Commision number 2.7.1.105: 6-phosphofructo-2-kinase.
3.1.3.46: fructose-2,6-bisphosphate 2-phosphatase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 AGS B A51 R52 G53 K54 T55 Y56 D130 S158 N169 Q172 V173 K174 V222 Y429 A13 R14 G15 K16 T17 Y18 D92 S120 N131 Q134 V135 K136 V184 Y391
Gene Ontology
Molecular Function
GO:0003824 catalytic activity
GO:0003873 6-phosphofructo-2-kinase activity
GO:0004331 fructose-2,6-bisphosphate 2-phosphatase activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0016301 kinase activity
GO:0016787 hydrolase activity
GO:0019900 kinase binding
GO:0042802 identical protein binding
GO:0070095 fructose-6-phosphate binding
Biological Process
GO:0006000 fructose metabolic process
GO:0006003 fructose 2,6-bisphosphate metabolic process
GO:0006094 gluconeogenesis
GO:0006096 glycolytic process
GO:0016310 phosphorylation
GO:0031100 animal organ regeneration
GO:0032868 response to insulin
GO:0033762 response to glucagon
GO:0042594 response to starvation
GO:0046835 carbohydrate phosphorylation
GO:0051384 response to glucocorticoid
GO:0051591 response to cAMP
GO:1904539 negative regulation of glycolytic process through fructose-6-phosphate
GO:1904540 positive regulation of glycolytic process through fructose-6-phosphate
Cellular Component
GO:0005829 cytosol
GO:0043540 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase complex

View graph for
Molecular Function
View graph for
Biological Process
View graph for
Cellular Component
External links
PDB RCSB:1k6m, PDBe:1k6m, PDBj:1k6m
PDBsum1k6m
PubMed12379646
UniProtP16118|F261_HUMAN 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 1 (Gene Name=PFKFB1)

[Back to BioLiP]