Structure of PDB 1js4 Chain B

Receptor sequence

>1js4B (length=605) Species: 2021 (Thermobifida fusca)

EPAFNYAEALQKSMFFYEAQRSGKLPENNRVSWRGDSGLNDGADVGLDLT
GGWYDAGDHVKFGFPMAFTATMLAWGAIESPEGYIRSGQMPYLKDNLRWV
NDYFIKAHPSPNVLYVQVGDGDADHKWWGPAEVMPMERPSFKVDPSCPGS
DVAAETAAAMAASSIVFADDDPAYAATLVQHAKQLYTFADTYRGVYSDCV
PAGAFYNSWSGYQDELVWGAYWLYKATGDDSYLAKAEYEYDFLSTEQQTD
LRSYRWTIAWDDKSYGTYVLLAKETGKQKYIDDANRWLDYWTVGVNGQRV
PYSPGGMAVLDTWGALRYAANTAFVALVYAKVIDDPVRKQRYHDFAVRQI
NYALGDNPRNSSYVVGFGNNPPRNPHHRTAHGSWTDSIASPAENRHVLYG
ALVGGPGSPNDAYTDDRQDYVANEVATDYNAGFSSALAMLVEEYGGTPLA
DFPPTEEPDGPEIFVEAQINTPGTTFTEIKAMIRNQSGWPARMLDKGTFR
YWFTLDEGVDPADITVSSAYNQCATPEDVHHVSGDLYYVEIDCTGEKIFP
GGQSEHRREVQFRIAGGPGWDPSNDWSFQGIGNELAPAPYIVLYDDGVPV
WGTAP

3D structure

• PDB: 1js4 Structure and mechanism of endo/exocellulase E4 from Thermomonospora fusca.
• Chain: B
• Resolution: 2.0 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D55 D58 Y206 E424
• Catalytic site (residue number reindexed from 1): D55 D58 Y206 E424
• Enzyme Commision number: 3.2.1.4: cellulase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	BGC	B	W209 W260 Y318	W209 W260 Y318
BS02	BGC	B	W256 D261 D262	W256 D261 D262
BS03	BGC	B	H125 H376 R378 Y420 E424	H125 H376 R378 Y420 E424
BS04	CA	B	S210 G211 D214 E215 D261	S210 G211 D214 E215 D261
BS05	CA	B	T504 D506 D571 N574 D575	T504 D506 D571 N574 D575

Gene Ontology

Molecular Function

• GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds
• GO:0030246 carbohydrate binding
• GO:0030248 cellulose binding

Biological Process

• GO:0005975 carbohydrate metabolic process

External links

• PDB: RCSB:1js4, PDBe:1js4, PDBj:1js4
• PDBsum: 1js4
• PubMed: 9334746
• UniProt: P26221|GUN4_THEFU Endoglucanase E-4 (Gene Name=celD)