Structure of PDB 1jry Chain B

Receptor sequence

>1jryB (length=568) Species: 56812 (Shewanella frigidimarina)

ADNLAEFHVQNQECDSCHTPDGELSNDSLTYENTQCVSCHGTLAEVAETT
KHEHYNAHASHFPGEVACTSCHSAHEKSMVYCDSCHSFDFNMPYAKKWLR
DEPTIAELAKDKSERQAALASAPHDTVDVVVVGSGGAGFSAAISATDSGA
KVILIEKEPVIGGNAKLAAGGMNAAWTDQQKAKKITDSPELMFEDTMKGG
QNINDPALVKVLSSHSKDSVDWMTAMGADLTDVGMMGGASVNRAHRPTGG
AGVGAHVVQVLYDNAVKRNIDLRMNTRGIEVLKDDKGTVKGILVKGMYKG
YYWVKADAVILATGGFAKNNERVAKLDPSLKGFISTNQPGAVGDGLDVAE
NAGGALKDMQYIQAHPTLSVKGGVMVTEAVRGNGAILVNREGKRFVNEIT
TKDKASAAILAQTGKSAYLIFDDSVRKSLSKIDKYIGLGVAPTADSLVKL
GKMEGIDGKALTETVARYNSLVSSGKDTDFERPNLPRALNEGNYYAIEVT
PGVHHTMGGVMIDTKAEVMNAKKQVIPGLYGAGEVTGGVHGANRLGGNAI
SDIITFGRLAGEEAAKYS

3D structure

• PDB: 1jry Kinetic and crystallographic analysis of the key active site acid/base arginine in a soluble fumarate reductase.
• Chain: B
• Resolution: 2.0 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): M236 Q363 H365 M375 E378 R381 K402 H504 H505 R544
• Catalytic site (residue number reindexed from 1): M236 Q363 H365 M375 E378 R381 K402 H504 H505 R544
• Enzyme Commision number: 1.3.2.4: fumarate reductase (cytochrome).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	HEM	B	H8 V9 C14 C17 H18 L24 S73 A74 H75	H8 V9 C14 C17 H18 L24 S73 A74 H75
BS02	HEM	B	L4 F7 H8 Q12 S16 C17 C36 C39 H40 H72 Y94	L4 F7 H8 Q12 S16 C17 C36 C39 H40 H72 Y94
BS03	HEM	B	H40 L43 H52 A57 H58 C68 C71 H72 F90 N91 M92	H40 L43 H52 A57 H58 C68 C71 H72 F90 N91 M92
BS04	HEM	B	Y55 S60 H61 F62 C82 C85 H86 F88 L167 Q338 V374 K431 K434 Y435 L438	Y55 S60 H61 F62 C82 C85 H86 F88 L167 Q338 V374 K431 K434 Y435 L438
BS05	FAD	B	V132 G133 G135 A137 E156 K157 G163 N164 A165 L167 A168 A169 G170 G171 R277 G278 A312 T313 G314 Q338 D344 M375 H504 H505 G533 E534 R544 G547 N548 A549 I550 I553	V132 G133 G135 A137 E156 K157 G163 N164 A165 L167 A168 A169 G170 G171 R277 G278 A312 T313 G314 Q338 D344 M375 H504 H505 G533 E534 R544 G547 N548 A549 I550 I553
BS06	FUM	B	G170 M236 H365 M375 T377 E378 K402 R544 G546 G547	G170 M236 H365 M375 T377 E378 K402 R544 G546 G547

Gene Ontology

Molecular Function

• GO:0009055 electron transfer activity
• GO:0010181 FMN binding
• GO:0016491 oxidoreductase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0008202 steroid metabolic process
• GO:0009061 anaerobic respiration
• GO:0019645 anaerobic electron transport chain

Cellular Component

• GO:0030288 outer membrane-bounded periplasmic space
• GO:0042597 periplasmic space

External links

• PDB: RCSB:1jry, PDBe:1jry, PDBj:1jry
• PDBsum: 1jry
• PubMed: 11591148
• UniProt: P0C278|FCCA_SHEFR Fumarate reductase (cytochrome) (Gene Name=fccA)