Structure of PDB 1jgt Chain B

Receptor sequence
>1jgtB (length=500) Species: 1901 (Streptomyces clavuligerus) [Search protein sequence]
GAPVLPAAFGFLASARTGGGPGPVFATRGSHTDIDTPQGERSLAATLVHA
PSVAPDRAVARSLTGAPTTAVLAGEIYNRDELLSVLPAGPAPEGDAELVL
RLLERYDLHAFRLVNGRFATVVRTGDRVLLATDHAGSVPLYTCVAPGEVR
ASTEAKALAAHRDPKGFPLADARRVAGLTGVYQVPAGAVMDIDLGSGTAV
THRTWTPGLSRRILPEGEAVAAVRAALEKAVAQRVTPGDTPLVVLSGGID
SSGVAACAHRAAGELDTVSMGTDTSNEFREARAVVDHLRTRHREITIPTT
ELLAQLPYAVWASESVDPDIIEYLLPLTALYRALDGPERRILTGYGADIP
LGGMHREDRLPALDTVLAHDMATFDGLNEMSPVLSTLAGHWTTHPYWDRE
VLDLLVSLEAGLKRRHGRDKWVLRAAMADALPAETVNRPKLSGTTSSFSR
LLLDHGVAEDRVHEAKRQVVRELFDLTVGGGRHPSEVDTDDVVRSVADRT
3D structure
PDB1jgt Structure of beta-lactam synthetase reveals how to synthesize antibiotics instead of asparagine.
ChainB
Resolution1.95 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) A76 G77 D322 Y348 E382 K443
Catalytic site (residue number reindexed from 1) A73 G74 D319 Y345 E379 K440
Enzyme Commision number 6.3.3.4: (carboxyethyl)arginine beta-lactam-synthase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 MG B D253 D351 D250 D348
BS02 APC B V247 L248 S249 G251 I252 D253 S254 S272 M273 L330 G347 Y348 D351 K423 K443 V244 L245 S246 G248 I249 D250 S251 S269 M270 L327 G344 Y345 D348 K420 K440
BS03 CMA B Y326 Y348 G349 D351 I352 M357 D373 E382 Y323 Y345 G346 D348 I349 M354 D370 E379
Gene Ontology
Molecular Function
GO:0004066 asparagine synthase (glutamine-hydrolyzing) activity
GO:0005524 ATP binding
GO:0016874 ligase activity
GO:0034027 (carboxyethyl)arginine beta-lactam-synthase activity
GO:0046872 metal ion binding
Biological Process
GO:0006529 asparagine biosynthetic process
GO:0033050 clavulanic acid biosynthetic process
Cellular Component
GO:0005829 cytosol

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:1jgt, PDBe:1jgt, PDBj:1jgt
PDBsum1jgt
PubMed11473258
UniProtP0DJQ7|BLS_STRCL Carboxyethyl-arginine beta-lactam-synthase (Gene Name=bls)

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