Structure of PDB 1inh Chain B

Receptor sequence

>1inhB (length=388) Species: 11320 (Influenza A virus)

VEYRNWSKPQCQITGFAPFSKDNSIRLSAGGDIWVTREPYVSCDPVKCYQ
FALGQGTTLDNKHSNDTVHDRIPHRTLLMNELGVPFHLGTRQVCIAWSSS
SCHDGKAWLHVCITGDDKNATASFIYDGRLVDSIGSWSQNILRTQESECV
CINGTCTVVMTDGSASGRADTRILFIEEGKIVHISPLAGSAQHVEECSCY
PRYPGVRCICRDNWKGSNRPVVDINMEDYSIDSSYVCSGLVGDTPRNDDR
SSNSNCRDPNNERGTQGVKGWAFDNGNDLWMGRTISKDLRSGYETFKVIG
GWSTPNSKSQINRQVIVDSDNRSGYSGIFSVEGKSCINRCFYVELIRGRK
QETRVWWTSNSIVVFCGTSGTYGTGSWPDGANINFMPI

3D structure

• PDB: 1inh Structure-based inhibitors of influenza virus sialidase. A benzoic acid lead with novel interaction.
• Chain: B
• Resolution: 2.4 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D151 E277 R292 R371 Y406
• Catalytic site (residue number reindexed from 1): D70 E196 R211 R290 Y325
• Enzyme Commision number: 3.2.1.18: exo-alpha-sialidase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	MAN	B	F377 K378 Q391 I392 N393 R394	F296 K297 Q310 I311 N312 R313
BS02	BMA	B	W361 I392 N393 R394 Q395 Y453	W280 I311 N312 R313 Q314 Y372
BS03	FUC	B	N146 D147	N65 D66
BS04	CA	B	D293 G297 D324 G345 T346 Q347	D212 G216 D243 G264 T265 Q266
BS05	ST6	B	R118 E119 D151 W178 E276 E277 Y406	R37 E38 D70 W97 E195 E196 Y325

Gene Ontology

Molecular Function

• GO:0004308 exo-alpha-sialidase activity

Biological Process

• GO:0005975 carbohydrate metabolic process
• GO:0046761 viral budding from plasma membrane

Cellular Component

• GO:0016020 membrane
• GO:0033644 host cell membrane
• GO:0055036 virion membrane

External links

• PDB: RCSB:1inh, PDBe:1inh, PDBj:1inh
• PDBsum: 1inh
• PubMed: 7650674
• UniProt: P06820|NRAM_I67A0 Neuraminidase (Gene Name=NA)