Structure of PDB 1iaw Chain B

Receptor sequence

>1iawB (length=304) Species: 68170 (Lentzea aerocolonigenes)

EPDDDLERVRATLYSLDPDGDRTAGVLRDTLDQLYDGQRTGRWNFDQLHK
TEKTHMGTLVEINLHREFQFGDGFETDYEIAGVQVDCKFSMSQGAWMLPP
ESIGHICLVIWASDQQCAWTAGLVKVIPQFLGTANRDLKRRLTPEGRAQV
VKLWPDHGKLQENLLLHIPGDVRDQIFSAKSSRGNQHGQARVNELFRRVH
GRLIGRAVIATVAQQDDFMKRVRGSGGARSILRPEGIIILGHQDNDPKVA
NDLGLPVPRKGQVVAARVVPADEGDQRQTAEIQGRRWAVAVPGDPIVEAP
VVPR

3D structure

• PDB: 1iaw Structure of NaeI-DNA complex reveals dual-mode DNA recognition and complete dimer rearrangement.
• Chain: B
• Resolution: 2.4 Å

Enzymatic activity

• Enzyme Commision number: 3.1.21.4: type II site-specific deoxyribonuclease.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	B	Q195 G197 Q224 D225 D226 K229 R230 Q252	Q186 G188 Q215 D216 D217 K220 R221 Q243
BS02	dna	B	R215 K229 R232 S234 G235 R238 K269 G270	R206 K220 R223 S225 G226 R229 K260 G261
BS03	dna	B	K59 K62 T63 D86 K97 F98 S99 M100 M106 E110 D146 K148 R150	K50 K53 T54 D77 K88 F89 S90 M91 M97 E101 D137 K139 R141
BS04	dna	B	K59 T60 T142 N144 R145 R150 R156	K50 T51 T133 N135 R136 R141 R147

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0004519 endonuclease activity
• GO:0009036 type II site-specific deoxyribonuclease activity

Biological Process

• GO:0009307 DNA restriction-modification system

External links

• PDB: RCSB:1iaw, PDBe:1iaw, PDBj:1iaw
• PDBsum: 1iaw
• PubMed: 11473254
• UniProt: P50187|T2N1_LENAE Type II restriction enzyme NaeI (Gene Name=naeIR)