Structure of PDB 1ho5 Chain B

Receptor sequence
>1ho5B (length=525) Species: 562 (Escherichia coli) [Search protein sequence]
YEQDKTYKITVLHTNDHHGHFWRNEYGEYGLAAQKTLVDGIRKEVAAEGG
SVLLLSGGDINTGVPESDLQDAEPDFRGMNLVGYDAMAIGNHEFDNPLTV
LRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKRQDLKIAVIGLTTDDT
AKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHMGHYDNGE
HGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPC
KPDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTW
EDGKSERVLYTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDR
DKVRFVQTNMGRLILAAQMDRTGADFAVMSGGGIRDSIEAGDISYKNVLK
VQPFGNVVVYADMTGKEVIDYLTAVAQMKPDSGAYPQFANVSFVAKDGKL
NDLKIKGEPVDPAKTYRMATLNFNATGGDGYPRLDNKPGYVNTGFIDAEV
LKAYIQKSSPLDVSVYEPKGEVSWQ
3D structure
PDB1ho5 Mechanism of hydrolysis of phosphate esters by the dimetal center of 5'-nucleotidase based on crystal structures.
ChainB
Resolution2.1 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) D41 H43 D84 N116 H117 D120 H217 H252 Q254 R375 R379 R410
Catalytic site (residue number reindexed from 1) D16 H18 D59 N91 H92 D95 H192 H227 Q229 R350 R354 R385
Enzyme Commision number 3.1.3.5: 5'-nucleotidase.
3.6.1.45: UDP-sugar diphosphatase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 MN B D41 H43 D84 Q254 D16 H18 D59 Q229
BS02 MN B D84 N116 H217 H252 D59 N91 H192 H227
BS03 PO4 B N116 H117 H252 N91 H92 H227
BS04 ADN B I178 G407 R410 F429 N431 G458 F498 D504 I153 G382 R385 F404 N406 G433 F473 D479
Gene Ontology
Molecular Function
GO:0000166 nucleotide binding
GO:0008252 nucleotidase activity
GO:0008253 5'-nucleotidase activity
GO:0008768 UDP-sugar diphosphatase activity
GO:0016787 hydrolase activity
GO:0016788 hydrolase activity, acting on ester bonds
GO:0046872 metal ion binding
GO:0106411 XMP 5'-nucleosidase activity
Biological Process
GO:0009166 nucleotide catabolic process
Cellular Component
GO:0030288 outer membrane-bounded periplasmic space
GO:0042597 periplasmic space

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:1ho5, PDBe:1ho5, PDBj:1ho5
PDBsum1ho5
PubMed11491293
UniProtP07024|USHA_ECOLI Protein UshA (Gene Name=ushA)

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