Structure of PDB 1h9y Chain B

Receptor sequence

>1h9yB (length=519) Species: 82367 (Paracoccus pantotrophus)

LSDAQYNEANKIYFERCAGCHGVLRKGATGKALTPDLTRDLGFDYLQSFI
TYGSPAGMPNWGTSGELSAEQVDLMANYLLLDPAAPPEFGMKEMRESWKV
HVAPEDRPTQQENDWDLENLFSVTLRDAGQIALIDGATYEIKSVLDTGYA
VHISRLSASGRYLFVIGRDGKVNMIDLWMKEPTTVAEIKIGSEARSIETS
KMEGWEDKYAIAGAYWPPQYVIMDGETLEPKKIQSTRGMTYDEQEYHPEP
RVAAILASHYRPEFIVNVKETGKILLVDYTDLDNLKTTEISAERFLHDGG
LDGSHRYFITAANARNKLVVIDTKEGKLVAIEDTGGQTPHPGRGANFVHP
TFGPVWATSHMGDDSVALIGTDPEGHPDNAWKILDSFPALGGGSLFIKTH
PNSQYLYVDATLNPEAEISGSVAVFDIKAMTGDGSDPEFKTLPIAEWAGI
TEGQPRVVQGEFNKDGTEVWFSVWNGKDQESALVVVDDKTLELKHVIKDE
RLVTPTGKFNVYNTMTDTY

3D structure

• PDB: 1h9y The Structure of an Alternative Form of Paracoccus Pantotrophus Cytochrome Cd1 Nitrite Reductase
• Chain: B
• Resolution: 2.4 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): C65 C68 H69 M106 H345 H388
• Catalytic site (residue number reindexed from 1): C17 C20 H21 M58 H297 H340
• Enzyme Commision number: 1.7.2.1: nitrite reductase (NO-forming).
  1.7.99.1: hydroxylamine reductase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	HEC	B	R64 C65 C68 H69 G78 L81 Y93 F97 I98 S102 A104 G105 M106 P107 W109	R16 C17 C20 H21 G30 L33 Y45 F49 I50 S54 A56 G57 M58 P59 W61
BS02	DHE	B	R174 H200 I201 R216 R243 S244 Y263 A301 A302 H345 R391 F444 T554 F557	R126 H152 I153 R168 R195 S196 Y215 A253 A254 H297 R343 F396 T506 F509

Gene Ontology

Molecular Function

• GO:0009055 electron transfer activity
• GO:0016491 oxidoreductase activity
• GO:0020037 heme binding
• GO:0046872 metal ion binding
• GO:0050418 hydroxylamine reductase activity
• GO:0050421 nitrite reductase (NO-forming) activity

Cellular Component

• GO:0042597 periplasmic space

External links

• PDB: RCSB:1h9y, PDBe:1h9y, PDBj:1h9y
• PDBsum: 1h9y
• PubMed: 11373294
• UniProt: P72181|NIRS_PARPN Nitrite reductase (Gene Name=nirS)