Structure of PDB 1ges Chain B

Receptor sequence
>1gesB (length=449) Species: 562 (Escherichia coli) [Search protein sequence]
TKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVNVGCVPKK
VMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYEN
VLGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGV
EYGIDSDGFFALPALPERVAVVGAGYIGVELGGVINGLGAKTHLFEMFDA
PLPSFDPMISETLVEVMNAEGPQLHTNAIPKAVVKNTDGSLTLELEDGRS
ETVDCLIWAIGREPANDNINLEAAGVKTNEKGYIVVDKYQNTNIEGIYAV
GDNTGAVELTPVAVAAGRRLSERLFNNKPDEHLDYSNIPTVVFSHPPIGT
VGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMKLVCVGSEEK
IVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
3D structure
PDB1ges Anatomy of an engineered NAD-binding site.
ChainB
Resolution1.74 Å
3D
structure
Catalytic site residues are labeled in the structure
[Spin on]
[Spin off]
[Reset orientation]

[High quality]
[Low quality]

[White background]
[Black background]

[Download]
[Download structure with residue number starting from 1]
Enzymatic activity
Catalytic site (original residue number in PDB) L38 C42 C47 K50 Y177 E181 A437 H439 E444
Catalytic site (residue number reindexed from 1) L37 C41 C46 K49 Y176 E180 A436 H438 E443
Enzyme Commision number 1.8.1.7: glutathione-disulfide reductase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 FAD B I10 G11 G13 S14 G15 E34 A35 G40 T41 C42 G46 C47 K50 F114 A115 T139 G140 Y177 I178 R263 G302 D303 E309 L310 T311 I9 G10 G12 S13 G14 E33 A34 G39 T40 C41 G45 C46 K49 F113 A114 T138 G139 Y176 I177 R262 G301 D302 E308 L309 T310
Gene Ontology
Molecular Function
GO:0004362 glutathione-disulfide reductase (NADPH) activity
GO:0016491 oxidoreductase activity
GO:0016668 oxidoreductase activity, acting on a sulfur group of donors, NAD(P) as acceptor
GO:0050660 flavin adenine dinucleotide binding
GO:0050661 NADP binding
GO:0071949 FAD binding
Biological Process
GO:0006749 glutathione metabolic process
GO:0034599 cellular response to oxidative stress
GO:0045454 cell redox homeostasis
GO:0098869 cellular oxidant detoxification
Cellular Component
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0016020 membrane

View graph for
Molecular Function
View graph for
Biological Process
View graph for
Cellular Component
External links
PDB RCSB:1ges, PDBe:1ges, PDBj:1ges
PDBsum1ges
PubMed7833810
UniProtP06715|GSHR_ECOLI Glutathione reductase (Gene Name=gor)

[Back to BioLiP]