Structure of PDB 1ga2 Chain B

Receptor sequence

>1ga2B (length=613) Species: 1521 (Ruminiclostridium cellulolyticum)

GTYNYGEALQKSIMFYEFQRSGDLPADKRDNWRDDSGMKDGSDVGVDLTG
GWYDAGDHVKFNLPMSYTSAMLAWSLYEDKDAYDKSGQTKYIMDGIKWAN
DYFIKCNPTPGVYYYQVGDGGKDHSWWGPAEVMQMERPSFKVDASKPGSA
VCASTAASLASAAVVFKSSDPTYAEKCISHAKNLFDMADKAKSDAGYTAA
SGYYSSSSFYDDLSWAAVWLYLATNDSTYLDKAESYVPNWGKEQQTDIIA
YKWGQCWDDVHYGAELLLAKLTNKQLYKDSIEMNLDFWTTGVNGTRVSYT
PKGLAWLFQWGSLRHATTQAFLAGVYAEWEGCTPSKVSVYKDFLKSQIDY
ALGSTGRSFVVGYGVNPPQHPHHRTAHGSWTDQMTSPTYHRHTIYGALVG
GPDNADGYTDEINNYVNNEIACDYNAGFTGALAKMYKHSGGDPIPNFKAI
EKITNDEVIIKAGLNSTGPNYTEIKAVVYNQTGWPARVTDKISFKYFMDL
SEIVAAGIDPLSLVTSSNYSEGKNTKVSGVLPWDVSNNVYYVNVDLTGEN
IYPGGQSACRREVQFRIAAPQGTTYWNPKNDFSYDGLPTTSTVNTVTNIP
VYDNGVKVFGNEP

3D structure

• PDB: 1ga2 X-Ray Crystal Structure of the Multidomain Endoglucanase Cel9G from Clostridium cellulolyticum Complexed with Natural and Synthetic Cello-Oligosaccharides
• Chain: B
• Resolution: 1.7 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D55 D58 Y205 E420
• Catalytic site (residue number reindexed from 1): D54 D57 Y204 E419
• Enzyme Commision number: 3.2.1.4: cellulase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	BGC	B	W254 C257 D259	W253 C256 D258
BS02	BGC	B	W254 D259 D260	W253 D258 D259
BS03	CA	B	S209 D212 D213 D259	S208 D211 D212 D258
BS04	CA	B	D500 E503 N578 N581 D582	D499 E502 N577 N580 D581

Gene Ontology

Molecular Function

• GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds
• GO:0030246 carbohydrate binding
• GO:0030248 cellulose binding

Biological Process

• GO:0005975 carbohydrate metabolic process

External links

• PDB: RCSB:1ga2, PDBe:1ga2, PDBj:1ga2
• PDBsum: 1ga2
• PubMed: 12837787
• UniProt: P37700|GUNG_RUMCH Endoglucanase G (Gene Name=celCCG)