Structure of PDB 1fzi Chain B

Receptor sequence

>1fziB (length=512) Species: 414 (Methylococcus capsulatus)

AANRAPTSVNAQEVHRWLQSFNWDFKNNRTKYATKYKMANETKEQFKLIA
KEYARMEAVKDERQFGSLQDALTRLNAGVRVHPKWNETMKVVSNFLEVGE
YNAIAATGMLWDSAQAAEQKNGYLAQVLDEIRHTHQCAYVNYYFAKNGQD
PAGHNDARRTRTIGPLWKGMKRVFSDGFISGDAVECSLNLQLVGEACFTN
PLIVAVTEWAAANGDEITPTVFLSIETDELRHMANGYQTVVSIANDPASA
KYLNTDLNNAFWTQQKYFTPVLGMLFEYGSKFKVEPWVKTWDRWVYEDWG
GIWIGRLGKYGVESPRSLKDAKQDAYWAHHDLYLLAYALWPTGFFRLALP
DQEEMEWFEANYPGWYDHYGKIYEEWRARGCEDPSSGFIPLMWFIENNHP
IYIDRVSQVPFCPSLAKGASTLRVHEYNGEMHTFSDQWGERMWLAEPERY
ECQNIFEQYEGRELSEVIAELHGLRSDGKTLIAQPHVRGDKLWTLDDIKR
LNCVFKNPVKAF

3D structure

• PDB: 1fzi Xenon and halogenated alkanes track putative substrate binding cavities in the soluble methane monooxygenase hydroxylase.
• Chain: B
• Resolution: 3.3 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): E114 E144 H147 E209 E243 H246
• Catalytic site (residue number reindexed from 1): E100 E130 H133 E195 E229 H232
• Enzyme Commision number: 1.14.13.25: methane monooxygenase (soluble).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FE	B	E114 E144 H147	E100 E130 H133
BS02	FE	B	E144 E209 E243 H246	E130 E195 E229 H232
BS03	XE	B	L289 G293	L275 G279
BS04	XE	B	L353 L478 F519	L339 L464 F505

Gene Ontology

Molecular Function

• GO:0004497 monooxygenase activity
• GO:0005515 protein binding
• GO:0015049 methane monooxygenase [NAD(P)H] activity
• GO:0016491 oxidoreductase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0006730 one-carbon metabolic process

External links

• PDB: RCSB:1fzi, PDBe:1fzi, PDBj:1fzi
• PDBsum: 1fzi
• PubMed: 11297413
• UniProt: P22869|MEMA_METCA Methane monooxygenase component A alpha chain (Gene Name=mmoX)