Structure of PDB 1fz2 Chain B

Receptor sequence

>1fz2B (length=510) Species: 414 (Methylococcus capsulatus)

RAPTSVNAQEVHRWLQSFNWDFKNNRTKYATKYKMANETKEQFKLIAKEY
ARMEAVKDERQFGSLQDALTRLNAGVRVHPKWNETMKVVSNFLEVGEYNA
IAATGMLWDSAQAAEQKNGYLAQVLDEIRHTHQCAYVNYYFAKNGQDPAG
HNDARRTRTIGPLWKGMKRVFSDGFISGDAVECSLNLQLVGEACFTNPLI
VAVTEWAAANGDEITPTVFLSIETDELRHMANGYQTVVSIANDPASAKYL
NTDLNNAFWTQQKYFTPVLGMLFEYGSKFKVEPWVKTWDRWVYEDWGGIW
IGRLGKYGVESPRSLKDAKQDAYWAHHDLYLLAYALWPTGFFRLALPDQE
EMEWFEANYPGWYDHYGKIYEEWRARGCEDPSSGFIPLMWFIENNHPIYI
DRVSQVPFCPSLAKGASTLRVHEYNGEMHTFSDQWGERMWLAEPERYECQ
NIFEQYEGRELSEVIAELHGLRSDGKTLIAQPHVRGDKLWTLDDIKRLNC
VFKNPVKAFN

3D structure

• PDB: 1fz2 Crystal structures of the soluble methane monooxygenase hydroxylase from Methylococcus capsulatus (Bath) demonstrating geometrical variability at the dinuclear iron active site.
• Chain: B
• Resolution: 2.15 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): E114 E144 H147 E209 E243 H246
• Catalytic site (residue number reindexed from 1): E97 E127 H130 E192 E226 H229
• Enzyme Commision number: 1.14.13.25: methane monooxygenase (soluble).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FE2	B	E114 E144 H147	E97 E127 H130
BS02	FE2	B	E209 E243 H246	E192 E226 H229

Gene Ontology

Molecular Function

• GO:0004497 monooxygenase activity
• GO:0005515 protein binding
• GO:0015049 methane monooxygenase [NAD(P)H] activity
• GO:0016491 oxidoreductase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0006730 one-carbon metabolic process

External links

• PDB: RCSB:1fz2, PDBe:1fz2, PDBj:1fz2
• PDBsum: 1fz2
• PubMed: 11456616
• UniProt: P22869|MEMA_METCA Methane monooxygenase component A alpha chain (Gene Name=mmoX)