Structure of PDB 1fmc Chain B

Receptor sequence

>1fmcB (length=255) Species: 562 (Escherichia coli)

MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDINADAANH
VVDEIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGG
PKPFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAE
NKNINMTSYASSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSV
ITPEIEQKMLQHTPIRRLGQPQDIANAALFLCSPAASWVSGQILTVSGGG
VQELN

3D structure

• PDB: 1fmc Crystal structures of the binary and ternary complexes of 7 alpha-hydroxysteroid dehydrogenase from Escherichia coli.
• Chain: B
• Resolution: 1.8 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): G22 S146 M156 Y159 K163
• Catalytic site (residue number reindexed from 1): G22 S146 M156 Y159 K163
• Enzyme Commision number: 1.1.1.159: 7alpha-hydroxysteroid dehydrogenase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	CHO	B	G99 S146 A148 N151 M156 Y159 G190 A196 L197 Q252	G99 S146 A148 N151 M156 Y159 G190 A196 L197 Q252
BS02	NAI	B	G18 A21 I23 D42 I43 C67 D68 I69 N95 G97 I144 K163 P189 G190 I192 T194 A196	G18 A21 I23 D42 I43 C67 D68 I69 N95 G97 I144 K163 P189 G190 I192 T194 A196

Gene Ontology

Molecular Function

• GO:0008709 cholate 7-alpha-dehydrogenase activity
• GO:0016491 oxidoreductase activity
• GO:0042802 identical protein binding
• GO:0051287 NAD binding
• GO:0106281 chenodeoxycholate 7-alpha-dehydrogenase (NAD+) activity

Biological Process

• GO:0016042 lipid catabolic process
• GO:0030573 bile acid catabolic process

Cellular Component

• GO:0005829 cytosol
• GO:0032991 protein-containing complex

External links

• PDB: RCSB:1fmc, PDBe:1fmc, PDBj:1fmc
• PDBsum: 1fmc
• PubMed: 8672472
• UniProt: P0AET8|HDHA_ECOLI 7alpha-hydroxysteroid dehydrogenase (Gene Name=hdhA)