Structure of PDB 1fgj Chain B

Receptor sequence

>1fgjB (length=499) Species: 915 (Nitrosomonas europaea)

DISTVPDETYDALKLDRGKATPKETYEALVKRYKDPAHGAGKGTMGDYWE
PIAISIYMDPNTFYKPPVSPKEVAERKDCVECHSDETPVWVRAWKRSTHA
NLDKIRNLKSDDPLYYKKGKLEEVENNLRSMGKLGEKETLKEVGCIDCHV
DVNKKDKADHTKDIRMPTADTCGTCHLREFAERESERDTMVWPNGQWPAG
RPSHALDYTANIETTVWATMPQREVAEGCTMCHTNQNKCDNCHTRHEFSA
AESRKPEACATCHSGVDHNNWEAYTMSKHGKLAEMNRDKWNWEVRLKDAF
SKGGQNAPTCAACHMEYEGEYTHNITRKTRWANYPFVPGIAENITSDWSE
ARLDSWVLTCTQCHSERFARSYLDLMDKGTLEGLAKYQEANAIVHKMYED
GTLTGQKTNRPNPPEPEKPGFGIFTQLFWSKGNNPASLELKVLEMGENNL
AKMHVGLAHVNPGGWTYTEGWGPMNRAYVEIQDEYTKMQELSALQARVN

3D structure

• PDB: 1fgj The 2.8 A structure of hydroxylamine oxidoreductase from a nitrifying chemoautotrophic bacterium, Nitrosomonas europaea.
• Chain: B
• Resolution: 2.8 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): H233 D267 H268 Y334 Y467
• Catalytic site (residue number reindexed from 1): H233 D267 H268 Y334 Y467
• Enzyme Commision number: 1.7.2.6: hydroxylamine dehydrogenase.
  1.7.2.9: hydroxylamine oxidase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	HEM	B	Y57 Y64 P67 A74 C79 C82 H83 H149 H160 I164	Y57 Y64 P67 A74 C79 C82 H83 H149 H160 I164
BS02	HEM	B	Y57 H83 W90 H99 G144 C145 C148 H149 M166 P167 K238 D240 R245 H246 F248	Y57 H83 W90 H99 G144 C145 C148 H149 M166 P167 K238 D240 R245 H246 F248
BS03	HEM	B	T98 H99 L102 K117 K120 V143 C172 C175 H176 C239 H243 F248	T98 H99 L102 K117 K120 V143 C172 C175 H176 C239 H243 F248
BS04	HEC	B	W197 R201 A210 N211 T214 C229 C232 H233 C262 H263 H268 N333 F428	W197 R201 A210 N211 T214 C229 C232 H233 C262 H263 H268 N333 F428
BS05	HEM	B	Y116 K117 H176 E179 C239 C242 H243 S253 R254 R295 L296 M315 H323	Y116 K117 H176 E179 C239 C242 H243 S253 R254 R295 L296 M315 H323
BS06	HEM	B	P202 S203 H204 C232 H233 N235 N241 C259 C262 H263	P202 S203 H204 C232 H233 N235 N241 C259 C262 H263
BS07	HEM	B	H263 N270 W271 Y274 T309 C310 C313 H314 T329 R330 W331 W356 A458 H459	H263 N270 W271 Y274 T309 C310 C313 H314 T329 R330 W331 W356 A458 H459
BS08	HEM	B	H279 L282 N306 A307 P308 C360 C363 H364 F368 V460	H279 L282 N306 A307 P308 C360 C363 H364 F368 V460

Gene Ontology

Molecular Function

• GO:0016491 oxidoreductase activity
• GO:0020037 heme binding
• GO:0033740 hydroxylamine oxidoreductase activity
• GO:0042802 identical protein binding
• GO:0046872 metal ion binding
• GO:0047991 hydroxylamine oxidase activity

Biological Process

• GO:0019331 anaerobic respiration, using ammonium as electron donor
• GO:0070207 protein homotrimerization

Cellular Component

• GO:0044222 anammoxosome

External links

• PDB: RCSB:1fgj, PDBe:1fgj, PDBj:1fgj
• PDBsum: 1fgj
• PubMed: 9095195
• UniProt: Q50925|HAO_NITEU Hydroxylamine oxidoreductase (Gene Name=hao1)