Structure of PDB 1f0o Chain B

Receptor sequence
>1f0oB (length=152) Species: 585 (Proteus vulgaris) [Search protein sequence]
PDLNKLLELWPHIQEYQDLALKHGINDIFQDNGGKLLQVLLITGLTVLPE
GNDAVDNAGQEYELKSINIDLTKGFSTHHHMNPVIIAKYRQVPWIFAIYR
GIAIEAIYRLEPKDLEFYYDKWERKWYSDGHKDINNPKIPVKYVMEHGTK
IY
3D structure
PDB1f0o PvuII endonuclease contains two calcium ions in active sites.
ChainB
Resolution2.5 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) D58 E68 K70
Catalytic site (residue number reindexed from 1) D53 E63 K65
Enzyme Commision number 3.1.21.4: type II site-specific deoxyribonuclease.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna B D34 S71 L76 T77 S81 H84 N141 K143 D31 S66 L71 T72 S76 H79 N136 K138
BS02 dna B Q33 H84 K130 N140 N141 Q30 H79 K125 N135 N136
BS03 CA B G56 D58 E68 G51 D53 E63
BS04 CA B D58 E68 L69 K70 D53 E63 L64 K65
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0004519 endonuclease activity
GO:0009036 type II site-specific deoxyribonuclease activity
GO:0046872 metal ion binding
Biological Process
GO:0009307 DNA restriction-modification system

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Molecular Function
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Biological Process
External links
PDB RCSB:1f0o, PDBe:1f0o, PDBj:1f0o
PDBsum1f0o
PubMed10903853
UniProtP23657|T2P2_PROHU Type II restriction enzyme PvuII (Gene Name=pvuIIR)

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