Structure of PDB 1eyu Chain B

Receptor sequence
>1eyuB (length=153) Species: 585 (Proteus vulgaris) [Search protein sequence]
SHPDLNKLLELWPHIQEYQDLALKHGINDIFQDNGGKLLQVLLITGLTVL
PGNDAVDNAGQEYELKSINIDLTKGFSTHHHMNPVIIAKYRQVPWIFAIY
RGIAIEAIYRLEPKDLEFYYDKWERKWYSDGHKDINNPKIPVKYVMEHGT
KIY
3D structure
PDB1eyu PvuII endonuclease contains two calcium ions in active sites.
ChainB
Resolution1.78 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) D58 E68 K70
Catalytic site (residue number reindexed from 1) D54 E64 K66
Enzyme Commision number 3.1.21.4: type II site-specific deoxyribonuclease.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna B D34 N35 G56 N57 S71 L76 S81 T82 H83 H84 N141 K143 D33 N34 G52 N53 S67 L72 S77 T78 H79 H80 N137 K139
BS02 dna B Q33 H84 Y123 K130 N140 N141 P145 Q32 H80 Y119 K126 N136 N137 P141
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0004519 endonuclease activity
GO:0009036 type II site-specific deoxyribonuclease activity
GO:0046872 metal ion binding
Biological Process
GO:0009307 DNA restriction-modification system

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Molecular Function
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Biological Process
External links
PDB RCSB:1eyu, PDBe:1eyu, PDBj:1eyu
PDBsum1eyu
PubMed10903853
UniProtP23657|T2P2_PROHU Type II restriction enzyme PvuII (Gene Name=pvuIIR)

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