Structure of PDB 1eqh Chain B

Receptor sequence

>1eqhB (length=551) Species: 9940 (Ovis aries)

VNPCCYYPCQHQGICVRFGLDRYQCDCTRTGYSGPNCTIPEIWTWLRTTL
RPSPSFIHFLLTHGRWLWDFVNATFIRDTLMRLVLTVRSNLIPSPPTYNI
AHDYISWESFSNVSYYTRILPSVPRDCPTPMGTKGKKQLPDAEFLSRRFL
LRRKFIPDPQGTNLMFAFFAQHFTHQFFKTSGKMGPGFTKALGHGVDLGH
IYGDNLERQYQLRLFKDGKLKYQMLNGEVYPPSVEEAPVLMHYPRGIPPQ
SQMAVGQEVFGLLPGLMLYATIWLREHNRVCDLLKAEHPTWGDEQLFQTA
RLILIGETIKIVIEEYVQQLSGYFLQLKFDPELLFGAQFQYRNRIAMEFN
QLYHWHPLMPDSFRVGPQDYSYEQFLFNTSMLVDYGVEALVDAFSRQPAG
RIGGGRNIDHHILHVAVDVIKESRVLRLQPFNEYRKRFGMKPYTSFQELT
GEKEMAAELEELYGDIDALEFYPGLLLEKCHPNSIFGESMIEMGAPFSLK
GLLGNPICSPEYWKASTFGGEVGFNLVKTATLKKLVCLNTKTCPYVSFHV
P

3D structure

• PDB: 1eqh Structural analysis of NSAID binding by prostaglandin H2 synthase: time-dependent and time-independent inhibitors elicit identical enzyme conformations.
• Chain: B
• Resolution: 2.7 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): Q203 H207 L384 Y385 H388 G526 S530
• Catalytic site (residue number reindexed from 1): Q171 H175 L352 Y353 H356 G494 S498
• Enzyme Commision number: 1.14.99.1: prostaglandin-endoperoxide synthase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	HEM	B	A202 Q203 H207 F210 T212 L295 N382 H386 H388 M391 I444 V447 D450	A170 Q171 H175 F178 T180 L263 N350 H354 H356 M359 I412 V415 D418
BS02	FLP	B	V116 R120 V349 L352 Y355 Y385 W387 I523 G526 A527 S530	V84 R88 V317 L320 Y323 Y353 W355 I491 G494 A495 S498	BindingDB: IC50=150nM,Ki=1000nM

Gene Ontology

Molecular Function

• GO:0004601 peroxidase activity
• GO:0020037 heme binding

Biological Process

• GO:0006979 response to oxidative stress

External links

• PDB: RCSB:1eqh, PDBe:1eqh, PDBj:1eqh
• PDBsum: 1eqh
• PubMed: 11318639
• UniProt: P05979|PGH1_SHEEP Prostaglandin G/H synthase 1 (Gene Name=PTGS1)