Structure of PDB 1ee2 Chain B

Receptor sequence

>1ee2B (length=373) Species: 9796 (Equus caballus)

STAGKVIKCKAAVLWEQKKPFSIEEVEVAPPKAHEVRIKMVAAGICRSDD
HVVSGTLVAPLPVIAGHEAAGIVESIGEGVTTVRPGDKVIPLFIPQCGKC
SVCKHPEGNLCLKNLSMPRGTMQDGTSRFTCRGKPIHHFLGTSTFSQYTV
VDEISVAKIDAASPLEKVCLVGCGFSTGYGSAVKVAKVTQGSTCAVFGLG
GVGLSVIMGCKAAGAARIIGVDINKDKFAKAKEVGATECVNPQDYKKPIQ
EVLTEMSNGGVDFSFEVIGRLDTMVAALSCCQEAYGVSVIVGVPPDSQNL
SMNPMLLLSGRTWKGAIFGGFKSKDSVPKLVADFMAKKFALDPLITHVLP
FEKINEGFDLLRSGKSIRTILTF

3D structure

• PDB: 1ee2 Structural basis for substrate specificity differences of horse liver alcohol dehydrogenase isozymes.
• Chain: B
• Resolution: 1.54 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): C46 R47 S48 H51 H67 E68 C97 C100 C103 C111 C173 T177 R368
• Catalytic site (residue number reindexed from 1): C46 R47 S48 H51 H67 E68 C97 C100 C103 C111 C173 T177 R368
• Enzyme Commision number: 1.1.1.1: alcohol dehydrogenase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ZN	B	C46 H67 C173	C46 H67 C173
BS02	ZN	B	C97 C100 C103 C111	C97 C100 C103 C111
BS03	NAD	B	C46 R47 S48 H51 C173 T177 G200 V202 D222 I223 I268 V291 G292 V293 I317	C46 R47 S48 H51 C173 T177 G200 V202 D222 I223 I268 V291 G292 V293 I317
BS04	CHD	B	S48 L57 S116 I317	S48 L57 S116 I317

Gene Ontology

Molecular Function

• GO:0004022 alcohol dehydrogenase (NAD+) activity
• GO:0004745 all-trans-retinol dehydrogenase (NAD+) activity
• GO:0008270 zinc ion binding
• GO:0016491 oxidoreductase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0042572 retinol metabolic process
• GO:0042573 retinoic acid metabolic process

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol

External links

• PDB: RCSB:1ee2, PDBe:1ee2, PDBj:1ee2
• PDBsum: 1ee2
• PubMed: 11041853
• UniProt: P00328|ADH1S_HORSE Alcohol dehydrogenase S chain