Structure of PDB 1e0y Chain B

Receptor sequence

>1e0yB (length=550) Species: 69488 (Penicillium simplicissimum) [Search protein sequence]

EFRPLTLPPKLSLSDFNEFIQDIIRIVGSENVEVISVDGSYMKPTHTHDP
HHVMDQDYFLASAIVAPRNVADVQSIVGLANKFSFPLWPISIGRNSGYGG
AAPRVSGSVVLDMGKNMNRVLEVNVEGAYCVVEPGVTYHDLHNYLEANNL
RDKLWLDVPSLGGGSVLGNAVERGVGYTPYGDHWMMHSGMEVVLANGELL
RTGMGALPDPKRPETMGLKPEDQPWSKIAHLFPYGFGPYIDGLFSQSNMG
IVTKIGIWLMPNPGGYQSYLITLPKDGDLKQAVDIIRPLRLGMALQNVPT
IRHILLDAAVLGDKRSYSSRTEPLSDEELDKIAKQLNLGRWNFYGALYGP
EPIRRVLWETIKDAFSAIPGVKFYFPEDTPENSVLRVRDKTMQGIPTYDE
LKWIDWLPNGAHLFFSPIAKVSGEDAMMQYAVTKKRCQEAGLDFIGEFTV
GMREMHHIVCIVFNKKDLIQKRKVQWLMRTLIDDCAANGWGEYRTHLAFM
DQIMETYNWNNSSFLRFNEVLKNAVDPNGIIAPGKSGVWPSQYSHVTWKL

3D structure

PDB

1e0y Inversion of Stereospecificity in Vanillyl-Alcohol Oxidase

Chain

Resolution

2.75 Å

3D
structure

Catalytic site residues are labeled in the structure
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Enzymatic activity

Catalytic site (original residue number in PDB)	Y108 S170 E182 D192 D317 V397 E410 I414 H422 H466 Y503 R504 K545
Catalytic site (residue number reindexed from 1)	Y98 S160 E172 D182 D307 V387 E400 I404 H412 H456 Y493 R494 K535
Enzyme Commision number	1.1.3.38: vanillyl-alcohol oxidase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FAD	B	P99 S101 I102 G103 R104 N105 P169 S170 G174 S175 N179 E182 G184 V185 Y187 V262 W413 I414 H422 F424 R504	P89 S91 I92 G93 R94 N95 P159 S160 G164 S165 N169 E172 G174 V175 Y177 V252 W403 I404 H412 F414 R494
BS02	FCR	B	Y187 F424 E457 I468 Y503	Y177 F414 E447 I458 Y493

Gene Ontology

	Molecular Function
GO:0003824	catalytic activity
GO:0004458	D-lactate dehydrogenase (cytochrome) activity
GO:0008720	D-lactate dehydrogenase activity
GO:0016491	oxidoreductase activity
GO:0018465	vanillyl-alcohol oxidase activity
GO:0050660	flavin adenine dinucleotide binding
GO:0071949	FAD binding

	Biological Process
GO:0015945	methanol metabolic process
GO:1903457	lactate catabolic process

	Cellular Component
GO:0005737	cytoplasm
GO:0005739	mitochondrion
GO:0005777	peroxisome

View graph for
Molecular Function

View graph for
Biological Process

View graph for
Cellular Component

External links

PDB	RCSB:1e0y, PDBe:1e0y, PDBj:1e0y
PDBsum	1e0y
PubMed	10920192
UniProt	P56216\|VAOX_PENSI Vanillyl-alcohol oxidase (Gene Name=VAOA)

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