Structure of PDB 1djg Chain B

Receptor sequence

>1djgB (length=561) Species: 10116 (Rattus norvegicus)

NKMNFKELKDFLKELNIQVDDGYARKIFRECDHSQTDSLEDEEIETFYKM
LTQRAEIDRAFEEAAGSAETLSVERLVTFLQHQQREEEAGPALALSLIER
YEPSETAKAQRQMTKDGFLMYLLSADGNAFSLAHRRVYQDMDQPLSHYLV
SSSHNTYLLEDQLTGPSSTEAYIRALCKGCRCLELDCWDGPNQEPIIYHG
YTFTSKILFCDVLRAIRDYAFKASPYPVILSLENHCSLEQQRVMARHLRA
ILGPILLDQPLDGVTTSLPSPEQLKGKILLKGKKLGGLDKLKLVPELSDM
IIYCKSVHFGGFSSPGTSGQAFYEMASFSESRALRLLQESGNGFVRHNVS
CLSRIYPAGWRTDSSNYSPVEMWNGGCQIVALNFQTPGPEMDVYLGCFQD
NGGCGYVLKPAFLRDPNTTFNSRALTQGPWWRPERLRVRIISGQQLPKVN
KNKNSIVDPKVIVEIHGVGRDTGSRQTAVITNNGFNPRWDMEFEFEVTVP
DLALVRFMVEDYDSSSKNDFIGQSTIPWNSLKQGYRHVHLLSKNGDQHPS
ATLFVKISIQD

3D structure

• PDB: 1djg A ternary metal binding site in the C2 domain of phosphoinositide-specific phospholipase C-delta1.
• Chain: B
• Resolution: 2.6 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): H311 N312 E341 D343 H356 E390
• Catalytic site (residue number reindexed from 1): H154 N155 E184 D186 H199 E233
• Enzyme Commision number: 3.1.4.11: phosphoinositide phospholipase C.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	LA	B	N312 D343 E390	N155 D186 E233
BS02	LA	B	I651 D653 N677	I456 D458 N482
BS03	LA	B	D653 D706 Y707 D708	D458 D511 Y512 D513
BS04	LA	B	D706 D708 D714	D511 D513 D519

Gene Ontology

Molecular Function

• GO:0004435 phosphatidylinositol phospholipase C activity
• GO:0005509 calcium ion binding
• GO:0008081 phosphoric diester hydrolase activity

Biological Process

• GO:0006629 lipid metabolic process
• GO:0007165 signal transduction
• GO:0035556 intracellular signal transduction

External links

• PDB: RCSB:1djg, PDBe:1djg, PDBj:1djg
• PDBsum: 1djg
• PubMed: 9062102
• UniProt: P10688|PLCD1_RAT 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-1 (Gene Name=Plcd1)