Structure of PDB 1dew Chain B

Receptor sequence
>1dewB (length=279) Species: 9606 (Homo sapiens) [Search protein sequence]
EGPALYEDPPDHKTSPSGKPATLKICSWNVDGLRAWIKKKGLDWVKEEAP
DILCLQETKCSENKLPAELQELPGLSHQYWSAPSDKEGYSGVGLLSRQCP
LKVSYGIGDEEHDQEGRVIVAEFDSFVLVTAYVPNAGRGLVRLEYRQRWD
EAFRKFLKGLASRKPLVLCGDLNVAHEEIDLRNPKGNKKNAGFTPQERQG
FGELLQAVPLADSFRHLYPNTPYAYTFWTYMMNARSKNVGWRLDYFLLSH
SLLPALCDSKIRSKALGSDHCPITLYLAL
3D structure
PDB1dew DNA-bound structures and mutants reveal abasic DNA binding by APE1 and DNA repair coordination
ChainB
Resolution2.65 Å
3D
structure
Catalytic site residues are labeled in the structure
[Spin on]
[Spin off]
[Reset orientation]

[High quality]
[Low quality]

[White background]
[Black background]

[Download]
[Download structure with residue number starting from 1]
Enzymatic activity
Catalytic site (original residue number in PDB) D70 E96 N212 D283 D308
Catalytic site (residue number reindexed from 1) D31 E57 N173 D244 D269
Enzyme Commision number 3.1.11.2: exodeoxyribonuclease III.
3.1.21.-
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna B E96 Y128 Y171 N174 R177 D210 N212 N222 N226 F266 T268 M271 K276 V278 W280 H309 E57 Y89 Y132 N135 R138 D171 N173 N183 N187 F227 T229 M232 K237 V239 W241 H270
BS02 dna B D70 G71 R73 A74 K78 E126 Y269 M271 D31 G32 R34 A35 K39 E87 Y230 M232
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0003824 catalytic activity
GO:0004518 nuclease activity
GO:0004519 endonuclease activity
Biological Process
GO:0006281 DNA repair

View graph for
Molecular Function
View graph for
Biological Process
External links
PDB RCSB:1dew, PDBe:1dew, PDBj:1dew
PDBsum1dew
PubMed10667800
UniProtP27695|APEX1_HUMAN DNA repair nuclease/redox regulator APEX1 (Gene Name=APEX1)

[Back to BioLiP]