Structure of PDB 1de8 Chain B

Receptor sequence
>1de8B (length=276) Species: 9606 (Homo sapiens) [Search protein sequence]
ALYEDPPDHKTSPSGKPATLKICSWNVDGLRAWIKKKGLDWVKEEAPDIL
CLQETKCSENKLPAELQELPGLSHQYWSAPSDKEGYSGVGLLSRQCPLKV
SYGIGDEEHDQEGRVIVAEFDSFVLVTAYVPNAGRGLVRLEYRQRWDEAF
RKFLKGLASRKPLVLCGDLNVAHEEIDLRNPKGNKKNAGFTPQERQGFGE
LLQAVPLADSFRHLYPNTPYAYTFWTYMMNARSKNVGWRLDYFLLSHSLL
PALCDSKIRSKALGSDHCPITLYLAL
3D structure
PDB1de8 DNA-bound structures and mutants reveal abasic DNA binding by APE1 and DNA repair coordination [corrected
ChainB
Resolution2.95 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) D70 E96 N212 D283 D308
Catalytic site (residue number reindexed from 1) D28 E54 N170 D241 D266
Enzyme Commision number 3.1.11.2: exodeoxyribonuclease III.
3.1.21.-
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna B Y128 Y171 N174 R177 D210 N212 N222 N226 N229 A230 F266 T268 M271 V278 W280 L282 H309 Y86 Y129 N132 R135 D168 N170 N180 N184 N187 A188 F224 T226 M229 V236 W238 L240 H267
BS02 dna B D70 G71 A74 K78 E126 G127 Y269 M270 M271 D28 G29 A32 K36 E84 G85 Y227 M228 M229
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0003824 catalytic activity
GO:0004518 nuclease activity
GO:0004519 endonuclease activity
Biological Process
GO:0006281 DNA repair

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Molecular Function
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Biological Process
External links
PDB RCSB:1de8, PDBe:1de8, PDBj:1de8
PDBsum1de8
PubMed10667800
UniProtP27695|APEX1_HUMAN DNA repair nuclease/redox regulator APEX1 (Gene Name=APEX1)

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