Structure of PDB 1ddr Chain B
Receptor sequence
| >1ddrB (length=159) Species: 37762 (Escherichia coli B) [Search protein sequence] |
| MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGRHTWESI GRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVY EQFLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHS YCFEILERR |
3D structure
| PDB | 1ddr The effect of denaturants on protein structure. | ||
| Chain | B | ||
| Resolution | 2.45 Å | ||
| 3D structure |
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Enzymatic activity
| Catalytic site (original residue number in PDB) | I5 M20 W22 D27 L28 F31 L54 I91 T113 |
| Catalytic site (residue number reindexed from 1) | I5 M20 W22 D27 L28 F31 L54 I91 T113 |
| Enzyme Commision number | 1.5.1.3: dihydrofolate reductase. |
Interaction with ligand
| Site # |
Ligand | Ligand chain |
Binding residues on receptor (original residue number in PDB) |
Binding residues on receptor (residue number reindexed from 1) |
Binding affinity |
|---|---|---|---|---|---|
| BS01 | MTX | B | I5 A6 D27 L28 F31 I50 R52 R57 I94 | I5 A6 D27 L28 F31 I50 R52 R57 I94 | BindingDB: IC50=8.8nM,EC50=1nM |
| BS02 | URE | B | K58 N59 R71 | K58 N59 R71 |
Gene Ontology
| Molecular Function | |
|---|---|
| GO:0004146 | dihydrofolate reductase activity |
| GO:0005515 | protein binding |
| GO:0005542 | folic acid binding |
| GO:0016491 | oxidoreductase activity |
| GO:0050661 | NADP binding |
| GO:0051870 | methotrexate binding |
| GO:0051871 | dihydrofolic acid binding |
| GO:0070401 | NADP+ binding |
| GO:0070402 | NADPH binding |
