Structure of PDB 1d8w Chain B

Receptor sequence

>1d8wB (length=402) Species: 562 (Escherichia coli)

TTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDDVSGFEYP
GKARNASELRADLEQAMRLIPGPKRLNLHAIYLESDTPVSRDQIKPEHFK
NWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWIDHCKASR
RVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKL
NPAHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPT
EVISDKISAAMLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHD
LFDRVHIGLDFFDASINRIAAWVIGTRNMKKALLRALLEPTAELRKLEAP
GDYTARLALLEEQKSLPWQAVWEMYCQRHDTPAGSEWLESVRAYEKEILS
RR

3D structure

• PDB: 1d8w The structure of rhamnose isomerase from Escherichia coli and its relation with xylose isomerase illustrates a change between inter and intra-subunit complementation during evolution.
• Chain: B
• Resolution: 1.6 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): W193 E234 K236 D267 H270 H294 D302 D304 D334
• Catalytic site (residue number reindexed from 1): W169 E210 K212 D243 H246 H270 D278 D280 D310
• Enzyme Commision number: 5.3.1.14: L-rhamnose isomerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ZN	B	E234 D267 H294 D334	E210 D243 H270 D310

Gene Ontology

Molecular Function

• GO:0008270 zinc ion binding
• GO:0008740 L-rhamnose isomerase activity
• GO:0016853 isomerase activity
• GO:0030145 manganese ion binding
• GO:0033296 rhamnose binding
• GO:0042802 identical protein binding
• GO:0046872 metal ion binding

Biological Process

• GO:0019299 rhamnose metabolic process
• GO:0019301 rhamnose catabolic process
• GO:0019324 L-lyxose metabolic process
• GO:0051289 protein homotetramerization

Cellular Component

• GO:0005737 cytoplasm
• GO:0032991 protein-containing complex

External links

• PDB: RCSB:1d8w, PDBe:1d8w, PDBj:1d8w
• PDBsum: 1d8w
• PubMed: 10891278
• UniProt: P32170|RHAA_ECOLI L-rhamnose isomerase (Gene Name=rhaA)