Structure of PDB 1cnz Chain B

Receptor sequence

>1cnzB (length=363)

MSKNYHIAVLPGDGIGPEVMAQALKVMDAVRSRFDMRITTSHYDVGGIAI
DNHGHPLPKATVEGCEQADAILFGSVGGPKWENLPPESQPERGALLPLRK
HFKLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPK
GREGSGQYEKAFDTEVYHRFEIERIARIAFESARKRRRKVTSIDKANVLQ
SSILWREIVNDVAKTYPDVELAHMYIDNATMQLIKDPSQFDVLLCSNLFG
DILSDECAMITGSMGMLPSASLNEQGFGLYEPAGGSAPDIAGKNIANPIA
QILSLALLLRYSLDANDAATAIEQAINRALEEGVRTGDLARGAAAVSTDE
MGDIIARYVAEGV

3D structure

• PDB: 1cnz Crystal Structures of Eschericia Coli and Salmonella Typhimurium 3- Isopropylmalate Dehydrogenase and Comparison with Their Thermophilic Counterpart from Thermus Thermophilus
• Chain: B
• Resolution: 1.76 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): Y145 K195 D227 D251
• Catalytic site (residue number reindexed from 1): Y145 K195 D227 D251
• Enzyme Commision number: 1.1.1.85: 3-isopropylmalate dehydrogenase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	MN	B	D251 D255	D251 D255

Gene Ontology

Molecular Function

• GO:0000287 magnesium ion binding
• GO:0003862 3-isopropylmalate dehydrogenase activity
• GO:0016491 oxidoreductase activity
• GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
• GO:0046872 metal ion binding
• GO:0051287 NAD binding

Biological Process

• GO:0009098 L-leucine biosynthetic process

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol

External links

• PDB: RCSB:1cnz, PDBe:1cnz, PDBj:1cnz
• PDBsum: 1cnz
• PubMed: 9086278
• UniProt: P37412|LEU3_SALTY 3-isopropylmalate dehydrogenase (Gene Name=leuB)