Structure of PDB 1ckm Chain B

Receptor sequence
>1ckmB (length=317) Species: 10506 (Paramecium bursaria Chlorella virus 1) [Search protein sequence]
NITTERAVLTLNGLQIKLHKVVGESRDDIVAKMKDLAMDDHKFPRLPGPN
PVSIERKDFEKLKQNKYVVSEKTDGIRFMMFFTRVFGFKVCTIIDRAMTV
YLLPFKNIPRVLFQGSIFDGELCVDIVEKKFAFVLFDAVVVSGVTVSQMD
LASRFFAMKRSLKEFKNVPEDPAILRYKEWIPLEHPTIIKDHLKKANAIY
HTDGLIIMSVDEPVIYGRNFNLFKLKPGTHHTIDFIIMSEDGTIGIFDPN
LRKNVPVGKLDGYYNKGSIVECGFADGTWKYIQGRSDKNQANDRLTYEKT
LLNIEENITIDELLDLF
3D structure
PDB1ckm X-ray crystallography reveals a large conformational change during guanyl transfer by mRNA capping enzymes.
ChainB
Resolution2.5 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 2.7.7.50: mRNA guanylyltransferase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 GTP B K82 T83 R87 R106 E131 F146 K188 I216 R228 K234 K236 D244 R295 K72 T73 R77 R96 E121 F136 K178 I206 R218 K224 K226 D234 R285
Gene Ontology
Molecular Function
GO:0003824 catalytic activity
GO:0004484 mRNA guanylyltransferase activity
GO:0005524 ATP binding
GO:0005525 GTP binding
GO:0016779 nucleotidyltransferase activity
Biological Process
GO:0006139 nucleobase-containing compound metabolic process
GO:0006370 7-methylguanosine mRNA capping

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Molecular Function
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Biological Process
External links
PDB RCSB:1ckm, PDBe:1ckm, PDBj:1ckm
PDBsum1ckm
PubMed9160746
UniProtQ84424|MCE_PBCV1 mRNA-capping enzyme (Gene Name=A103R)

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