Structure of PDB 1bua Chain B

Receptor sequence
>1buaB (length=229) Species: 562 (Escherichia coli) [Search protein sequence]
SLRSDLINALYDEDVCGIISAEGKIYPLGSDTKVLSTIFELFSRPIINKI
AEKHGYIVEEPKQQNHYPDFTLYKPSEPNKKIAIDIKTTYTNKIKFTLGG
YTSFIRNNTKNIVYPFDQYIAHWIIGYVYTRSLKTYNINELNEIPKPYKG
VKVFLQDKWVIAGDLAGSGNTTNIGSIHAHYKDFVEGKGIFDSEDEFLDY
WRNYERTSQLRNDKYNNISEYRNWIYRGR
3D structure
PDB1bua Structural and energetic origins of indirect readout in site-specific DNA cleavage by a restriction endonuclease.
ChainB
Resolution2.15 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 3.1.21.4: type II site-specific deoxyribonuclease.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna B N70 S183 G184 N185 S223 N65 S168 G169 N170 S208 PDBbind-CN: Kd=550nM
BS02 dna B N70 K92 T93 T94 Y95 T106 T111 S112 K119 S183 T186 N188 N65 K87 T88 T89 Y90 T97 T102 S103 K110 S168 T171 N173 PDBbind-CN: Kd=550nM
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0004519 endonuclease activity
GO:0009036 type II site-specific deoxyribonuclease activity
GO:0046872 metal ion binding
Biological Process
GO:0009307 DNA restriction-modification system

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Molecular Function
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Biological Process
External links
PDB RCSB:1bua, PDBe:1bua, PDBj:1bua
PDBsum1bua
PubMed10074946
UniProtP04390|T2E5_ECOLX Type II restriction enzyme EcoRV (Gene Name=ecoRVR)

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