Structure of PDB 1bsu Chain B

Receptor sequence
>1bsuB (length=229) Species: 562 (Escherichia coli) [Search protein sequence]
SLRSDLINALYDEDVCGIISAEGKIYPLGSDTKVLSTIFELFSRPIINKI
AEKHGYIVEEPKQQNHYPDFTLYKPSEPNKKIAIDIKTTYTNIKFTLGGY
TSFIRNNTKNIVYPFDQYIAHWIIGYVYTRVSLKTYNINELNEIPKPYKG
VKVFLQDKWVIAGDLAGSGNTTNIGSIHAHYKDFVEGKGIFDSEDEFLDY
WRNYERTSQLRNDKYNNISEYRNWIYRGR
3D structure
PDB1bsu Structural and energetic origins of indirect readout in site-specific DNA cleavage by a restriction endonuclease.
ChainB
Resolution2.0 Å
3D
structure
[Spin on]
[Spin off]
[Reset orientation]

[High quality]
[Low quality]

[White background]
[Black background]

[Download]
[Download structure with residue number starting from 1]
Enzymatic activity
Enzyme Commision number 3.1.21.4: type II site-specific deoxyribonuclease.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna B Q69 N70 S183 G184 N185 S223 R226 Q64 N65 S168 G169 N170 S208 R211 PDBbind-CN: Kd=230nM
BS02 dna B T37 N70 K92 T93 T94 Y95 T106 G109 T111 S112 S183 T186 N188 T32 N65 K87 T88 T89 Y90 T96 G99 T101 S102 S168 T171 N173 PDBbind-CN: Kd=230nM
BS03 CA B D74 D90 D69 D85
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0004519 endonuclease activity
GO:0009036 type II site-specific deoxyribonuclease activity
GO:0046872 metal ion binding
Biological Process
GO:0009307 DNA restriction-modification system

View graph for
Molecular Function
View graph for
Biological Process
External links
PDB RCSB:1bsu, PDBe:1bsu, PDBj:1bsu
PDBsum1bsu
PubMed10074946
UniProtP04390|T2E5_ECOLX Type II restriction enzyme EcoRV (Gene Name=ecoRVR)

[Back to BioLiP]