Structure of PDB 1bss Chain B

Receptor sequence
>1bssB (length=228) Species: 562 (Escherichia coli) [Search protein sequence]
SLRSDLINALYDVCGIISAEGKIYPLGSDTKVLSTIFELFSRPIINKIAE
KHGYIVEEPKQQNHYPDFTLYKPSEPNKKIAIDIKATYTNKIKFTLGGYT
SFIRNNTKNIVYPFDQYIAHWIIGYVYTRSLKTYNINELNEIPKPYKGVK
VFLQDKWVIAGDLAGSGNTTNIGSIHAHYKDFVEGKGIFDSEDEFLDYWR
NYERTSQLRNDKYNNISEYRNWIYRGRK
3D structure
PDB1bss Metal ion-mediated substrate-assisted catalysis in type II restriction endonucleases
ChainB
Resolution2.15 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 3.1.21.4: type II site-specific deoxyribonuclease.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna B Q69 N70 S183 G184 N185 S223 Q62 N63 S166 G167 N168 S206
BS02 dna B N70 K92 T94 Y95 T106 T111 S112 K119 N120 S183 T186 N188 N63 K85 T87 Y88 T95 T100 S101 K108 N109 S166 T169 N171
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0004519 endonuclease activity
GO:0009036 type II site-specific deoxyribonuclease activity
GO:0046872 metal ion binding
Biological Process
GO:0009307 DNA restriction-modification system

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Molecular Function
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Biological Process
External links
PDB RCSB:1bss, PDBe:1bss, PDBj:1bss
PDBsum1bss
PubMed9811827
UniProtP04390|T2E5_ECOLX Type II restriction enzyme EcoRV (Gene Name=ecoRVR)

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