Structure of PDB 1bir Chain B

Receptor sequence

>1birB (length=104) Species: 5062 (Aspergillus oryzae)

ACDYTCGSNCYSSSDVSTAQAAGYKLHEDGETVGSNSYPHKYNNYEGFDF
SVSSPYYEWPILSSGDVYSGGSPGADRVVFNENNQLAGVITHTGASGNNA
VECT

3D structure

• PDB: 1bir A catalytic function for the structurally conserved residue Phe 100 of ribonuclease T1.
• Chain: B
• Resolution: 1.8 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): Y38 H40 E58 D76 R77 H92 A100
• Catalytic site (residue number reindexed from 1): Y38 H40 E58 D76 R77 H92 A100
• Enzyme Commision number: 4.6.1.24: ribonuclease T1.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	2GP	B	Y38 H40 Y42 N43 N44 Y45 E46 E58 R77 H92 N98	Y38 H40 Y42 N43 N44 Y45 E46 E58 R77 H92 N98	MOAD: Kd=138uM

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0004519 endonuclease activity
• GO:0004521 RNA endonuclease activity
• GO:0004540 RNA nuclease activity
• GO:0016829 lyase activity
• GO:0046589 ribonuclease T1 activity

Biological Process

• GO:0008150 biological_process

Cellular Component

• GO:0001411 hyphal tip
• GO:0030428 cell septum

External links

• PDB: RCSB:1bir, PDBe:1bir, PDBj:1bir
• PDBsum: 1bir
• PubMed: 8844843
• UniProt: P00651|RNT1_ASPOR Guanyl-specific ribonuclease T1 (Gene Name=rntA)