Structure of PDB 1b94 Chain B

Receptor sequence
>1b94B (length=244) Species: 562 (Escherichia coli) [Search protein sequence]
SLRSDLINALYDENQKYDVCGIISAEGKIYPLGSDTKVLSTIFELFSRPI
INKIAEKHGYIVEEPKQQNHYPDFTLYKPSEPNKKIAIDIKTTYTNKENE
KIKFTLGGYTSFIRNNTKNIVYPFDQYIAHWIIGYVYTRVATRKSSLKTY
NINELNEIPKPYKGVKVFLQDKWVIAGDLAGSGNTTNIGSIHAHYKDFVE
GKGIFDSEDEFLDYWRNYERTSQLRNDKYNNISEYRNWIYRGRK
3D structure
PDB1b94 Structural analysis of a mutational hot-spot in the EcoRV restriction endonuclease: a catalytic role for a main chain carbonyl group.
ChainB
Resolution1.9 Å
3D
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Enzymatic activity
Enzyme Commision number 3.1.21.4: type II site-specific deoxyribonuclease.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna B K38 Q68 Q69 N70 S183 G184 N185 T222 S223 K37 Q67 Q68 N69 S182 G183 N184 T221 S222
BS02 dna B T37 N70 K92 T93 T94 Y95 G109 T111 S112 K119 N120 S183 T186 N188 T36 N69 K91 T92 T93 Y94 G108 T110 S111 K118 N119 S182 T185 N187
BS03 CA B D74 D90 D73 D89
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0004519 endonuclease activity
GO:0009036 type II site-specific deoxyribonuclease activity
GO:0046872 metal ion binding
Biological Process
GO:0009307 DNA restriction-modification system

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Molecular Function
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Biological Process
External links
PDB RCSB:1b94, PDBe:1b94, PDBj:1b94
PDBsum1b94
PubMed10446231
UniProtP04390|T2E5_ECOLX Type II restriction enzyme EcoRV (Gene Name=ecoRVR)

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