Structure of PDB 1az0 Chain B

Receptor sequence
>1az0B (length=229) Species: 562 (Escherichia coli) [Search protein sequence]
SLRSDLINALYDEDVCGIISAEGKIYPLGSDTKVLSTIFELFSRPIINKI
AEKHGYIVEEPKQQNHYPDFTLYKPSEPNKKIAIDIKTTYTNIKFTLGGY
TSFIRNNTKNIVYPFDQYIAHWIIGYVYTRVSLKTYNIELNEIPKPYKGV
KVFLQDKWVIAGDLAGSGNTTNIGSIHAHYKDFVEGKGIFDSEDEFLDYW
RNYERTSQLRNDKYNNISEYRNWIYRGRK
3D structure
PDB1az0 Conformational transitions and structural deformability of EcoRV endonuclease revealed by crystallographic analysis.
ChainB
Resolution2.0 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 3.1.21.4: type II site-specific deoxyribonuclease.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna B Q69 N70 S183 G184 N185 S223 Q64 N65 S167 G168 N169 S207
BS02 dna B T37 N70 D90 K92 T93 T94 Y95 T106 G109 T111 S112 S183 T186 N188 T32 N65 D85 K87 T88 T89 Y90 T96 G99 T101 S102 S167 T170 N172
BS03 CA B D74 D90 D69 D85
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0004519 endonuclease activity
GO:0009036 type II site-specific deoxyribonuclease activity
GO:0046872 metal ion binding
Biological Process
GO:0009307 DNA restriction-modification system

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Molecular Function
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Biological Process
External links
PDB RCSB:1az0, PDBe:1az0, PDBj:1az0
PDBsum1az0
PubMed9367757
UniProtP04390|T2E5_ECOLX Type II restriction enzyme EcoRV (Gene Name=ecoRVR)

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