Structure of PDB 1aso Chain B

Receptor sequence

>1asoB (length=552) Species: 3665 (Cucurbita pepo var. melopepo)

SQIRHYKWEVEYMFWAPNCNENIVMGINGQFPGPTIRANAGDSVVVELTN
KLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGETFFYNFTVDNPGT
FFYHGHLGMQRSAGLYGSLIVDPPQGKKEPFHYDGEINLLLSDWWHQSIH
KQEVGLSSKPIRWIGEPQTILLNGRGQFDCSIAAKYDSNLEPCKLKGSES
CAPYIFHVSPKKTYRIRIASTTALAALNFAIGNHQLLVVEADGNYVQPFY
TSDIDIYSGESYSVLITTDQNPSENYWVSVGTRARHPNTPPGLTLLNYLP
NSVSKLPTSPPPQTPAWDDFDRSKNFTYRITAAMGSPKPPVKFNRRIFLL
NTQNVINGYVKWAINDVSLALPPTPYLGAMKYNLLHAFDQNPPPEVFPED
YDIDTPPTNEKTRIGNGVYQFKIGEVVDVILQNANMMKENLSETHPWHLH
GHDFWVLGYGDGKFSAEEESSLNLKNPPLRNTVVIFPYGWTAIRFVADNP
GVWAFHCHIEPHLHMGMGVVFAEGVEKVGRIPTKALACGGTAKSLINNPK
NP

3D structure

• PDB: 1aso X-ray structures and mechanistic implications of three functional derivatives of ascorbate oxidase from zucchini. Reduced, peroxide and azide forms.
• Chain: B
• Resolution: 2.2 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): H60 H62 H104 H106 H445 H448 H450 H506 C507 H508 I509 H512 M517
• Catalytic site (residue number reindexed from 1): H60 H62 H104 H106 H445 H448 H450 H506 C507 H508 I509 H512 M517
• Enzyme Commision number: 1.10.3.3: L-ascorbate oxidase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	CU	B	H445 C507 H512 M517	H445 C507 H512 M517
BS02	CU	B	H106 H450 H506	H106 H450 H506
BS03	CU	B	H62 H104 H508	H62 H104 H508
BS04	CU	B	H60 H448	H60 H448

Gene Ontology

Molecular Function

• GO:0005507 copper ion binding
• GO:0008447 L-ascorbate oxidase activity
• GO:0016491 oxidoreductase activity
• GO:0046872 metal ion binding

Cellular Component

• GO:0005576 extracellular region
• GO:0009506 plasmodesma

External links

• PDB: RCSB:1aso, PDBe:1aso, PDBj:1aso
• PDBsum: 1aso
• PubMed: 8478945
• UniProt: P37064|ASO_CUCPM L-ascorbate oxidase