Structure of PDB 1amu Chain B

Receptor sequence
>1amuB (length=508) Species: 1393 (Brevibacillus brevis) [Search protein sequence]
GTHEEEQYLFAVNNTKAEYPRDKTIHQLFEEQVSKRPNNVAIVCENEQLT
YHELNVKANQLARIFIEKGIGKDTLVGIMMEKSIDLFIGILAVLKAGGAY
VPIDIEYPKERIQYILDDSQARMLLTQKHLVHLIHNQFNGQVEIFEEDTI
KIREGTNLHVPSKSTDLAYVIYTNPKGTMLEHKGISNLKVFFENSLNVTE
KDRIGQFASISFDASVWEMFMALLTGASLYIILKDTINDFVKFEQYINQK
EITVITLPPTYVVHLDPERILSIQTLITAGSATSPSLVNKWKEKVTYINA
YGPTETTICATTWVATKETIGHSVPIGAPIQNTQIYIVDENLQLKSVGEA
GELCIGGEGLARGYWKRPELTSQKFVDNPFVPGEKLYKTGDQARWLSDGN
IEYLGRIDNQVKIRGHRVELEEVESILLKHMYISETAVSVHKDHQEQPYL
CAYFVSEKHIPLEQLRQFSSEELPTYMIPSYFIQLDKMPLTSNGKIDRKQ
LPEPDLTF
3D structure
PDB1amu Structural basis for the activation of phenylalanine in the non-ribosomal biosynthesis of gramicidin S.
ChainB
Resolution1.9 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) T190 L210 T326 E327 K434 R439 K517
Catalytic site (residue number reindexed from 1) T173 L188 T304 E305 K412 R417 K495
Enzyme Commision number 5.1.1.11: phenylalanine racemase (ATP-hydrolyzing).
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 MG B P325 E327 P303 E305
BS02 PHE B F234 D235 W239 A301 G324 I330 K517 F212 D213 W217 A279 G302 I308 K495
BS03 AMP B T190 G302 S303 A304 A322 Y323 T326 D413 Y425 K517 T173 G280 S281 A282 A300 Y301 T304 D391 Y403 K495
External links
PDB RCSB:1amu, PDBe:1amu, PDBj:1amu
PDBsum1amu
PubMed9250661
UniProtP0C061|GRSA_ANEMI Gramicidin S synthase 1 (Gene Name=grsA)

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