Structure of PDB 1a74 Chain B

Receptor sequence
>1a74B (length=162) Species: 5791 (Physarum polycephalum) [Search protein sequence]
ALTNAQILAVIDSWEETVGQFPVITHHVPLGGGLQGTLHCYEIPLAAPYG
VGFAKNGPTRWQYKRTINQVVHRWGSHTVPFLLEPDNINGKTCTASHLCH
NTRCHNPLHLCWESLDDNKGRNWCPGPNGGCVHAVVCLRQGPLYGPGATV
AGPQQRGSHFVV
3D structure
PDB1a74 DNA binding and cleavage by the nuclear intron-encoded homing endonuclease I-PpoI.
ChainB
Resolution2.2 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) N119
Catalytic site (residue number reindexed from 1) N118
Enzyme Commision number 3.1.-.-
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna B N57 R61 Q63 R74 G76 T95 A96 H98 L116 N119 N123 N56 R60 Q62 R73 G75 T94 A95 H97 L115 N118 N122
BS02 dna B A48 P49 V52 G53 K56 N57 K65 T67 A47 P48 V51 G52 K55 N56 K64 T66
BS03 ZN B C125 C132 H134 C138 C124 C131 H133 C137
BS04 ZN B C41 C100 C105 H110 C40 C99 C104 H109
Gene Ontology
Molecular Function
GO:0004519 endonuclease activity
Biological Process
GO:0006314 intron homing

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Molecular Function
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Biological Process
External links
PDB RCSB:1a74, PDBe:1a74, PDBj:1a74
PDBsum1a74
PubMed9665136
UniProtQ94702|PPO1_PHYPO Intron-encoded endonuclease I-PpoI

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