Structure of PDB 8cdr Chain Aa

Receptor sequence

>8cdrAa (length=816) Species: 4932 (Saccharomyces cerevisiae)

MVAFTVDQMRSLMDKVTNVRNMSVIAHVDHGKSTLTDSLVQRAGKSTAIS
LYSEMSDEDVKEIKQKTDGNSFLINLIDSPGHVDFSSEVTAALRVTDGAL
VVVDTIEGVCVQTETVLRQALGERIKPVVVINKVDRALLELQVSKEDLYQ
TFARTVESVNVIVSTYADEVLGDVQVYPARGTVAFGSGLHGWAFTIRQFA
TRYAKKFGVDKAKMMDRLWGDSFFNPKTKKWTNKDTDAEGKPLERAFNMF
ILDPIFRLFTAIMNFKKDEIPVLLEKLEIVLKGDEKDLEGKALLKVVMRK
FLPAADALLEMIVLHLPSPVTAQAYRAEQLYEGPADDANCIAIKNCDPKA
DLMLYVSKMVPTSDKGRFYAFGRVFAGTVKSGQKVRIQGPNYVPGKKDDL
FIKAIQRVVLMMGRFVEPIDDCPAGNIIGLVGIDQFLLKTGTLTTSETAH
NMKVMKFSVSPVVQVAVEVKNANDLPKLVEGLKRLSKSDPCVLTYMSESG
EHIVAGTGELHLEICLQDLEHDHAGVPLKISPPVVAYRETVESESSQTAL
SKSPNKHNRIYLKAEPIDEEVSLAIENGIINPRDDFKARARIMADDYGWD
VTDARKIWCFGPDGNGPNLVIDQTKAVQYLHEIKDSVVAAFQWATKEGPI
FGEEMRSVRVNILDVTLHADAIHRGGGQIIPTMRRATYAGFLLADPKIQE
PVFLVEIQCPEQAVGGIYSVLNKKRGQVVSEEQRPGTPLFTVKAYLPVNE
SFGFTGELRQATGGQAFPQMVFDHWSTLGSDPLDPTSKAGEIVLAARKRH
GMKEEVPGWQEYYDKL

3D structure

• PDB: 8cdr mRNA reading frame maintenance during eukaryotic ribosome translocation
• Chain: Aa
• Resolution: 2.04 Å

Enzymatic activity

• Enzyme Commision number: 3.6.5.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	Aa	H27 V28 H108 R162 W669 G703 E737 V740 Y744 V754 V755 F766 R785 Q786 G789 G790	H27 V28 H82 R136 W643 G677 E711 V714 Y718 V728 V729 F740 R759 Q760 G763 G764
BS02	rna	Aa	K578 H583 R585 H694 D696 X699 L842	K552 H557 R559 H668 D670 X673 L816
BS03	rna	Aa	D390 K651 Q654	D364 K625 Q628
BS04	GDP	Aa	V28 G31 K32 S33 T34 N158 K159 D161 S213 L215	V28 G31 K32 S33 T34 N132 K133 D135 S187 L189
BS05	SO1	Aa	Y521 S523 E524 A562 P727 F729 V774 F798 W801	Y495 S497 E498 A536 P701 F703 V748 F772 W775

Gene Ontology

Molecular Function

• GO:0003746 translation elongation factor activity
• GO:0003924 GTPase activity
• GO:0005515 protein binding
• GO:0005525 GTP binding
• GO:0016787 hydrolase activity
• GO:0019843 rRNA binding
• GO:0042802 identical protein binding
• GO:0043022 ribosome binding
• GO:0051087 protein-folding chaperone binding

Biological Process

• GO:0006412 translation
• GO:0006414 translational elongation
• GO:0045901 positive regulation of translational elongation
• GO:1990145 maintenance of translational fidelity

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8cdr, PDBe:8cdr, PDBj:8cdr
• PDBsum: 8cdr
• PubMed: 38030725
• UniProt: P32324|EF2_YEAST Elongation factor 2 (Gene Name=EFT1)