Structure of PDB 4v9k Chain AY

Receptor sequence

>4v9kAY (length=687) Species: 262724 (Thermus thermophilus HB27)

KVEYDLKRLRNIGIAAHIDAGKTTTTERILYYTGRIHKIGEVHEGAATMD
FMEQERERGITITAAVTTCFWKDHRINIIDTPGHVDFTIEVERSMRVLDG
AIVVFDSSQGVEPQSETVWRQAEKYKVPRIAFANKMDKTGADLWLVIRTM
QERLGARPVVMQLPIGREDTFSGIIDVLRMKAYTYGNDLGTDIREIPIPE
EYLDQAREYHEKLVEVAADFDENIMLKYLEGEEPTEEELVAAIRKGTIDL
KITPVFLGSALKNKGVQLLLDAVVDYLPSPLDIPPIKGTTPEGEVVEIHP
DPNGPLAALAFKIMADPYVGRLTFIRVYSGTLTSGSYVYNTTKGRKERVA
RLLRMHANHREEVEELKAGDLGAVVGLKETITGDTLVGEDAPRVILESIE
VPEPVIDVAIEPKTKADQEKLSQALARLAEEDPTFRVSTHPETGQTIISG
MGELHLEIIVDRLKREFKVDANVGKPQVAYRETITKPVDVEGKFIRQTGG
RGQYGHVKIKVEPLPRGSGFEFVNAIVGGVIPKEYIPAVQKGIEEAMQSG
PLIGFPVVDIKVTLYDGSYHEVDSSEMAFKIAGSMAIKEAVQKGDPVILE
PIMRVEVTTPEEYMGDVIGDLNARRGQILGMEPRGNAQVIRAFVPLAEMF
GYATDLRSKTQGRGSFVMFFDHYQEVPKQVQEKLIKG

3D structure

• PDB: 4v9k Crystal structures of EF-G-ribosome complexes trapped in intermediate states of translocation.
• Chain: AY
• Resolution: 3.5 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	AY	Y321 V322 G323 Y340 R351 R354 K381 T501 G502 R504 K536	Y318 V319 G320 Y337 R348 R351 K378 T498 G499 R501 K533
BS02	rna	AY	I21 D89 Q117 K141 R499 Q506 M580 M617 I621 N625 A626 M634 R660 S661 Q664	I18 D86 Q114 K138 R496 Q503 M577 M614 I618 N622 A623 M631 R657 S658 Q661
BS03	GNP	AY	D22 G24 K25 T26 T27 I63 T64 N137 K138	D19 G21 K22 T23 T24 I60 T61 N134 K135
BS04	MG	AY	T26 T64	T23 T61

Gene Ontology

Molecular Function

• GO:0003746 translation elongation factor activity
• GO:0003924 GTPase activity
• GO:0005525 GTP binding

Biological Process

• GO:0006412 translation
• GO:0006414 translational elongation
• GO:0032790 ribosome disassembly

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol

External links

• PDB: RCSB:4v9k, PDBe:4v9k, PDBj:4v9k
• PDBsum: 4v9k
• PubMed: 23812722
• UniProt: Q5SHN5|EFG_THET8 Elongation factor G (Gene Name=fusA)