Structure of PDB 4v90 Chain AY

Receptor sequence

>4v90AY (length=686) Species: 300852 (Thermus thermophilus HB8)

KVEYDLKRLRNIGIAAHIDAGKTTTTERILYYTGRIHKIGEVHEGAATMD
FMEQERERGITITAAVTTCFWKDHRINIIDTPGHVDFTIEVERSMRVLDG
AIVVFDSSQGVEPQSETVWRQAEKYKVPRIAFANKMDKTGADLWLVIRTM
QERLGARPVVMQLPIGREDTFSGIIDVLRMKAYTYGNDLGTDIREIPIPE
EYLDQAREYHEKLVEVAADFDENIMLKYLEGEEPTEEELVAAIRKGTIDL
KITPVFLGSALKNKGVQLLLDAVVDYLPSPLDIPPIKGTTPEGEVVEIHP
DPNGPLAALAFKIMADPYVGRLTFIRVYSGTLTSGSYVYNTTKGRKERVA
RLLRMHANHREEVEELKAGDLGAVVGLKETITGDTLVGEDAPRVILESIE
VPEPVIDVAIEPKTKADQEKLSQALARLAEEDPTFRVSTHPETGQTIISG
MGELHLEIIVDRLKREFKVDANVGKPQVAYRETITKPVDVEGKFIRQTGG
RGQYGHVKIKVEPLPRGSGFEFVNAIVGGVIPKEYIPAVQKGIEEAMQSG
PLIGFPVVDIKVTLYDGSYHEVDSSEMAFKIAGSMAIKEAVQKGDPVILE
PIMRVEVTTPEEYMGDVIGDLNARRGQILGMEPRGNAQVIRAFVPLAEMF
GYATDLRSKTQGRGSFVMFFDHYQEVPKQVQEKLIK

3D structure

• PDB: 4v90 Structure of EF-G-ribosome complex in a pretranslocation state.
• Chain: AY
• Resolution: 2.95 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	AY	Y321 G323 Y340 K349 R351 A353 R354 K381 R430 R499 G505 S578	Y318 G320 Y337 K346 R348 A350 R351 K378 R427 R496 G502 S575
BS02	rna	AY	H20 I21 R61 H87 E456 R499 E614 M617 G618 G622 A626 I631 L632 G633 M634 R660 S661 Q664	H17 I18 R58 H84 E453 R496 E611 M614 G615 G619 A623 I628 L629 G630 M631 R657 S658 Q661
BS03	MG	AY	T26 T64	T23 T61

Gene Ontology

Molecular Function

• GO:0003746 translation elongation factor activity
• GO:0003924 GTPase activity
• GO:0005525 GTP binding

Biological Process

• GO:0006412 translation
• GO:0006414 translational elongation
• GO:0032790 ribosome disassembly

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol

External links

• PDB: RCSB:4v90, PDBe:4v90, PDBj:4v90
• PDBsum: 4v90
• PubMed: 23912278
• UniProt: Q5SHN5|EFG_THET8 Elongation factor G (Gene Name=fusA)