Structure of PDB 4wf1 Chain AT

Receptor sequence
>4wf1AT (length=85) Species: 83333 (Escherichia coli K-12) [Search protein sequence]
NIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDKAAAQKAFN
EMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA
3D structure
PDB4wf1 Negamycin induces translational stalling and miscoding by binding to the small subunit head domain of the Escherichia coli ribosome.
ChainAT
Resolution3.09 Å
3D
structure
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Enzymatic activity
Enzyme Commision number ?
Interaction with ligand
Site
#
Ligand Ligand
chain
Binding residues on receptor
(original residue number in PDB)
Binding residues on receptor
(residue number reindexed from 1)
Binding affinity
BS01 rna AT N3 K5 K9 R10 S14 A17 R18 H20 N21 S23 R24 R25 S26 M27 R29 T30 K33 Q55 P56 R60 K64 N70 K71 R74 K76 A77 N78 T80 Q82 N1 K3 K7 R8 S12 A15 R16 H18 N19 S21 R22 R23 S24 M25 R27 T28 K31 Q53 P54 R58 K62 N68 K69 R72 K74 A75 N76 T78 Q80
Gene Ontology
Molecular Function
GO:0003723 RNA binding
GO:0003735 structural constituent of ribosome
GO:0008073 ornithine decarboxylase inhibitor activity
GO:0019843 rRNA binding
GO:0070181 small ribosomal subunit rRNA binding
Biological Process
GO:0000028 ribosomal small subunit assembly
GO:0002181 cytoplasmic translation
GO:0006412 translation
Cellular Component
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0005840 ribosome
GO:0015935 small ribosomal subunit
GO:0022627 cytosolic small ribosomal subunit
GO:1990904 ribonucleoprotein complex

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Molecular Function

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Cellular Component
External links
PDB RCSB:4wf1, PDBe:4wf1, PDBj:4wf1
PDBsum4wf1
PubMed25368144
UniProtP0A7U7|RS20_ECOLI Small ribosomal subunit protein bS20 (Gene Name=rpsT)

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