Structure of PDB 7mpj Chain AR

Receptor sequence

>7mpjAR (length=188) Species: 4932 (Saccharomyces cerevisiae)

ANLRTQKRLAASVVGVGKRKVWLDPNETSEIAQANSRNAIRKLVKNGTIV
KKAVTVHSKSRTRAHAQSKREGRHSGYGKRKGTREARLPSQVVWIRRLRV
LRRLLAKYRDAGKIDKHLYHVLYKESKGNAFKHKRALVEHIIQAKADAQR
EKALNEEAEARRLKNRAARDRRAQRVAEKRDALLKEDA

3D structure

• PDB: 7mpj CryoEM structures of pseudouridine-free ribosome suggest impacts of chemical modifications on ribosome conformations.
• Chain: AR
• Resolution: 2.7 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	AR	E158 A159 R162 R163 R167 R170	E157 A158 R161 R162 R166 R169
BS02	rna	AR	N3 L4 R5 T6 K8 R9 L10 G18 K19 R20 K21 W23 L24 S37 R38 R42 K53 V55 T56 V57 H58 S59 K60 S61 R62 T63 R64 R74 H75 G77 Y78 G79 K80 R81 K82 G83 T84 R85 E86 A87 R88 L89 P90 Q92 W95 I96 R97 R100 V101 R103 R104 R110 K117 H118 Y120 H121 Y124 K125 K128 G129 N130 H134 K135	N2 L3 R4 T5 K7 R8 L9 G17 K18 R19 K20 W22 L23 S36 R37 R41 K52 V54 T55 V56 H57 S58 K59 S60 R61 T62 R63 R73 H74 G76 Y77 G78 K79 R80 K81 G82 T83 R84 E85 A86 R87 L88 P89 Q91 W94 I95 R96 R99 V100 R102 R103 R109 K116 H117 Y119 H120 Y123 K124 K127 G128 N129 H133 K134
BS03	MG	AR	K82 G83	K81 G82
BS04	MG	AR	R74 H75	R73 H74
BS05	MG	AR	H75 S76 G77	H74 S75 G76
BS06	MG	AR	R38 N39	R37 N38

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7mpj, PDBe:7mpj, PDBj:7mpj
• PDBsum: 7mpj
• PubMed: 35489333
• UniProt: P0CX82|RL19A_YEAST Large ribosomal subunit protein eL19A (Gene Name=RPL19A)