Structure of PDB 7mpj Chain AQ

Receptor sequence

>7mpjAQ (length=185) Species: 4932 (Saccharomyces cerevisiae)

GIDHTSKQHKRSGHRTAPKSDNVYLKLLVKLYTFLARRTDAPFNKVVLKA
LFLSKINRPPVSVSRIARALKQEGAANKTVVVVGTVTDDARIFEFPKTTV
AALRFTAGARAKIVKAGGECITLDQLAVRAPKGQNTLILRGPRNSREAVR
HFGMGPHKGKAPRILSTGRKFERARGRRRSKGFKV

3D structure

• PDB: 7mpj CryoEM structures of pseudouridine-free ribosome suggest impacts of chemical modifications on ribosome conformations.
• Chain: AQ
• Resolution: 2.7 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	AQ	G2 I3 H5 T6 K8 Q9 H10 K11 R12 G14 H15 R16 K20 S21 F35 R38 R39 P43 F44 L54 S55 K56 I57 N58 P61 S63 S65 R66 R69 Q73 T86 T88 D89 D90 R92 R105 T107 A108 G134 N136 T137 I139 R141 P143 R144 N145 S146 R147 E148 A149 R151 F153 P157 H158 G160 A162 R164 L166 S167 R170 K171 F172 R176 R178 R179 R180 S181 K182 G183 F184 K185	G1 I2 H4 T5 K7 Q8 H9 K10 R11 G13 H14 R15 K19 S20 F34 R37 R38 P42 F43 L53 S54 K55 I56 N57 P60 S62 S64 R65 R68 Q72 T85 T87 D88 D89 R91 R104 T106 A107 G133 N135 T136 I138 R140 P142 R143 N144 S145 R146 E147 A148 R150 F152 P156 H157 G159 A161 R163 L165 S166 R169 K170 F171 R175 R177 R178 R179 S180 K181 G182 F183 K184
BS02	MG	AQ	R16 L54	R15 L53
BS03	MG	AQ	A149 H152 R164	A148 H151 R163
BS04	MG	AQ	L54 S55 N58	L53 S54 N57

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:0043232 intracellular non-membrane-bounded organelle
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7mpj, PDBe:7mpj, PDBj:7mpj
• PDBsum: 7mpj
• PubMed: 35489333
• UniProt: P0CX49|RL18A_YEAST Large ribosomal subunit protein eL18A (Gene Name=RPL18A)