Structure of PDB 7top Chain AL18

Receptor sequence

>7topAL18 (length=185) Species: 4932 (Saccharomyces cerevisiae)

GIDHTSKQHKRSGHRTAPKSDNVYLKLLVKLYTFLARRTDAPFNKVVLKA
LFLSKINRPPVSVSRIARALKQEGAANKTVVVVGTVTDDARIFEFPKTTV
AALRFTAGARAKIVKAGGECITLDQLAVRAPKGQNTLILRGPRNSREAVR
HFGMGPHKGKAPRILSTGRKFERARGRRRSKGFKV

3D structure

• PDB: 7top Ribosome inhibition by C9ORF72-ALS/FTD-associated poly-PR and poly-GR proteins revealed by cryo-EM.
• Chain: AL18
• Resolution: 2.4 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	AL18	G2 H5 S7 K8 Q9 H10 R12 G14 H15 R16 S21 K31 F35 R38 P43 F44 V47 K50 S55 K56 I57 S63 S65 R66 R69 Q73 E74 T86 T88 D89 D90 R92 I93 R105 T107 A108 N136 T137 I139 R141 R144 N145 S146 R147 E148 R151 F153 G154 P157 H158 G160 A162 R164 S167 R170 K171 F172 R174 R179 S181 K182 G183 F184	G1 H4 S6 K7 Q8 H9 R11 G13 H14 R15 S20 K30 F34 R37 P42 F43 V46 K49 S54 K55 I56 S62 S64 R65 R68 Q72 E73 T85 T87 D88 D89 R91 I92 R104 T106 A107 N135 T136 I138 R140 R143 N144 S145 R146 E147 R150 F152 G153 P156 H157 G159 A161 R163 S166 R169 K170 F171 R173 R178 S180 K181 G182 F183

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:0043232 intracellular non-membrane-bounded organelle
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7top, PDBe:7top, PDBj:7top
• PDBsum: 7top
• PubMed: 35589706
• UniProt: P0CX49|RL18A_YEAST Large ribosomal subunit protein eL18A (Gene Name=RPL18A)