Structure of PDB 4v6l Chain AK

Receptor sequence

>4v6lAK (length=178) Species: 83333 (Escherichia coli K-12)

PRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVYSALETLA
QRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAM
RWIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAF
AHYRWLSLRSFSHQAGASSKQPALGYLN

3D structure

• PDB: 4v6l Structural insights into cognate versus near-cognate discrimination during decoding.
• Chain: AK
• Resolution: 13.2 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	AK	P1 R2 R3 R4 I6 G7 Q8 R9 K10 I11 K24 N27 I28 L29 M30 D32 G33 K34 K35 S36 T37 A38 I41 K75 S76 R77 R78 V79 R91 R94 R101 K109 K113 S114 M115 Y153 W155 L156 S157 L158 R159	P1 R2 R3 R4 I6 G7 Q8 R9 K10 I11 K24 N27 I28 L29 M30 D32 G33 K34 K35 S36 T37 A38 I41 K75 S76 R77 R78 V79 R91 R94 R101 K109 K113 S114 M115 Y153 W155 L156 S157 L158 R159
BS02	rna	AK	G81 S82 Y84 A149 F150 A151 W155 L158 R159 S160 F161 S162 G166 A167 S168	G81 S82 Y84 A149 F150 A151 W155 L158 R159 S160 F161 S162 G166 A167 S168

Gene Ontology

Molecular Function

• GO:0000049 tRNA binding
• GO:0003723 RNA binding
• GO:0003729 mRNA binding
• GO:0003735 structural constituent of ribosome
• GO:0005515 protein binding
• GO:0019843 rRNA binding

Biological Process

• GO:0000028 ribosomal small subunit assembly
• GO:0002181 cytoplasmic translation
• GO:0006412 translation
• GO:0017148 negative regulation of translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0015935 small ribosomal subunit
• GO:0016020 membrane
• GO:0022627 cytosolic small ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:4v6l, PDBe:4v6l, PDBj:4v6l
• PDBsum: 4v6l
• PubMed: 21378755
• UniProt: P02359|RS7_ECOLI Small ribosomal subunit protein uS7 (Gene Name=rpsG)