Structure of PDB 8uql Chain AF

Receptor sequence
>8uqlAF (length=82) Species: 562 (Escherichia coli) [Search protein sequence]
RVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEENDKTTVIALR
EIEEGLINNQILDVRERQEQQEQEAAELQAVT
3D structure
PDB8uql Escherichia coli transcription-translation coupled complex class A (TTC-A) containing RfaH bound to ops signal, mRNA with a 21 nt long spacer, and fMet-tRNAs in E-site and P-site of the ribosome
ChainAF
Resolution3.2 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 2.7.7.6: DNA-directed RNA polymerase.
Interaction with ligand
Site
#
Ligand Ligand
chain
Binding residues on receptor
(original residue number in PDB)
Binding residues on receptor
(residue number reindexed from 1)
Binding affinity
BS01 rna AF L80 V83 T84 L78 V81 T82
Gene Ontology
Molecular Function
GO:0001000 bacterial-type RNA polymerase core enzyme binding
GO:0003677 DNA binding
GO:0003899 DNA-directed 5'-3' RNA polymerase activity
GO:0016779 nucleotidyltransferase activity
GO:0034062 5'-3' RNA polymerase activity
Biological Process
GO:0006351 DNA-templated transcription
GO:0006352 DNA-templated transcription initiation
GO:0006879 intracellular iron ion homeostasis
GO:0009408 response to heat
GO:0031564 transcription antitermination
GO:0032784 regulation of DNA-templated transcription elongation
GO:0036460 cellular response to cell envelope stress
GO:0042128 nitrate assimilation
GO:0044780 bacterial-type flagellum assembly
GO:0048870 cell motility
GO:0065003 protein-containing complex assembly
GO:0071973 bacterial-type flagellum-dependent cell motility
GO:0090605 submerged biofilm formation
GO:2000142 regulation of DNA-templated transcription initiation
Cellular Component
GO:0000345 cytosolic DNA-directed RNA polymerase complex
GO:0000428 DNA-directed RNA polymerase complex
GO:0005829 cytosol
GO:0008023 transcription elongation factor complex
GO:0030880 RNA polymerase complex

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Molecular Function

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Biological Process

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Cellular Component
External links
PDB RCSB:8uql, PDBe:8uql, PDBj:8uql
PDBsum8uql
PubMed39117885
UniProtP0A800|RPOZ_ECOLI DNA-directed RNA polymerase subunit omega (Gene Name=rpoZ)

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