Structure of PDB 8ewc Chain AF

Receptor sequence

>8ewcAF (length=222) Species: 4932 (Saccharomyces cerevisiae)

AEQVAAERAARKAANKEKRAIILERNAAYQKEYETAERNIIQAKRDAKAA
GSYYVEAQHKLVFVVRIKGINKIPPKPRKVLQLLRLTRINSGTFVKVTKA
TLELLKLIEPYVAYGYPSYSTIRQLVYKRGFGKINKQRVPLSDNAIIEAN
LGKYGILSIDDLIHEIITVGPHFKQANNFLWPFKLSNPSGGWGVPRKFKH
FIQGGSFGNREEFINKLVKSMN

3D structure

• PDB: 8ewc Regulation of translation by ribosomal RNA pseudouridylation.
• Chain: AF
• Resolution: 2.45 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	AF	R30 R33 N37 R41 R60 R67 K70 K90 G91 I92 N93 K94 P97 K98 K101 L105 R107 R110 I111 N112 K121 A122 E125 Y141 S142 R145 K150 R151 K155 Q159 R160 K196 N200 K206 L207 S208 N209 P210 S211 W214 P217 R218 K219 F220 K241	R8 R11 N15 R19 R38 R45 K48 K68 G69 I70 N71 K72 P75 K76 K79 L83 R85 R88 I89 N90 K99 A100 E103 Y119 S120 R123 K128 R129 K133 Q137 R138 K174 N178 K184 L185 S186 N187 P188 S189 W192 P195 R196 K197 F198 K219
BS02	rna	AF	K128 R218 F220 K221 H222 I224 Q225	K106 R196 F198 K199 H200 I202 Q203

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0000463 maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0000470 maturation of LSU-rRNA
• GO:0002181 cytoplasmic translation
• GO:0042273 ribosomal large subunit biogenesis

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8ewc, PDBe:8ewc, PDBj:8ewc
• PDBsum: 8ewc
• PubMed: 37595043
• UniProt: P05737|RL7A_YEAST Large ribosomal subunit protein uL30A (Gene Name=RPL7A)