Structure of PDB 6hiv Chain AF

Receptor sequence

>6hivAF (length=442) Species: 5702 (Trypanosoma brucei brucei)

SSIFSPRYDWRTSGVHDIAPRDEGDFLYQGPQHVLPGAHPLPLHHPHNTI
TRPVISPYIPSPQRSHPYFTAPLPELPHFSTTKPIVYTYGTMKERIIAPV
FNLKNEVIYTRELDPFIFGMYPEVEELSKNLTYWMVRCQNFASKWDYETR
EIWRKAKKNWPNTGMGMPRVGNRKNHLYTWGGRTKPSKPWNMLMPTMDVK
TWSKSNRMMLTLKMLQGRLQVVDRLTLEEPTQECYLELCRNMSWDVRHTG
GGVLFMDGGSRITPSSEFDRAFFFGSFFNGRNKIVRPTVLCDEQYDYNKT
AAKQRMKGPKGAKNPIPINRFNAYDAMKHDRLVITEGALMQLEDELYEHK
LQILPPHIRNQLPEYGYLDSEALGDCVPSLKTIQMEAAARTEEAESDMYK
SFIDNPYNPWKDNMDASYAVDGADGTVQKFVDGKKVSWSMLS

3D structure

• PDB: 6hiv Evolutionary shift toward protein-based architecture in trypanosomal mitochondrial ribosomes.
• Chain: AF
• Resolution: 7.8 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	AF	S18 F21 S22 W27 R28 R38 H61 Y106 E140 E142 E143 K146 M152 A159 S160 W162 Y164 E165 T166 R167 K175 R186 G188 N192 H193 L194 Y195 T196 R200 T201 K202 K217 K221 R224 R298 Y314 A319 K320 R322 M323 K327 Y341 K345 H366 I370	S1 F4 S5 W10 R11 R21 H44 Y89 E123 E125 E126 K129 M135 A142 S143 W145 Y147 E148 T149 R150 K158 R169 G171 N175 H176 L177 Y178 T179 R183 T184 K185 K200 K204 R207 R281 Y297 A302 K303 R305 M306 K310 Y324 K328 H349 I353
BS02	peptide	AF	T317 A318	T300 A301
BS03	NAD	AF	D421 W455 M457 L458	D404 W438 M440 L441

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:6hiv, PDBe:6hiv, PDBj:6hiv
• PDBsum: 6hiv
• PubMed: 30213880
• UniProt: A0A1G4HYD1