Structure of PDB 8bqs Chain AD

Receptor sequence

>8bqsAD (length=441) Species: 312017 (Tetrahymena thermophila SB210)

SYGNLKDQDRIFTNLYRDGDPFVKGALKRGDWHQTKEILSNGPEWIIDEI
KKSGLRGRGGAGFLSGLKYSFMPKVNPDGRPSYLVINSDESEPGTCKDRE
ILRNDPHKLVEGALVVGFSMRARAAYIYIRGEFWVEANILQQAIDEAYAK
GFIGKNACGSGYDFDVYIHRGAGAYICGEETGLIESIEGKAGQPRVKPPF
PANAGLYGCPTTVTNVETVAVCPTIMRRGASWFASFGRPNNAGTKLYCIS
GHVNNPCTVEEEMSIPLRELLEKHCGGVRGGWDNLLAVIPGGSSVPMMPK
NVCDDVLMDFDALKAVGSGLGTAAVIVMDKSTDPIDAILRLSKFYKHESC
GQCTPCREGTGWIVDVMERLLVGNADYAEIDMLQQVTQQIEMHTICALGD
AAAWPVQGLIKNFREEIEDRIDSYHAKHPQLKKSRKSNPQI

3D structure

• PDB: 8bqs Structural basis of mitochondrial membrane bending by I-II-III2-IV2 supercomplex
• Chain: AD
• Resolution: 2.9 Å

Enzymatic activity

• Enzyme Commision number: 7.1.1.2: NADH:ubiquinone reductase (H(+)-translocating).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	SF4	AD	I206 C380 G381 Q382 C383 C386 T424 C426 L428	I176 C350 G351 Q352 C353 C356 T394 C396 L398
BS02	FMN	AD	G87 G89 N117 D119 Y205 G208 E209 E210 N245 L428	G57 G59 N87 D89 Y175 G178 E179 E180 N215 L398

Gene Ontology

Molecular Function

• GO:0008137 NADH dehydrogenase (ubiquinone) activity
• GO:0010181 FMN binding
• GO:0046872 metal ion binding
• GO:0051287 NAD binding
• GO:0051539 4 iron, 4 sulfur cluster binding

Biological Process

• GO:0006120 mitochondrial electron transport, NADH to ubiquinone
• GO:1902600 proton transmembrane transport

Cellular Component

• GO:0005739 mitochondrion
• GO:0005743 mitochondrial inner membrane
• GO:0045271 respiratory chain complex I

External links

• PDB: RCSB:8bqs, PDBe:8bqs, PDBj:8bqs
• PDBsum: 8bqs
• UniProt: Q23KE4